Molecular studies of co-formulated strains of the entomopathogenic fungus, Beauveria bassiana.
Molecular studies of co-formulated strains of the entomopathogenic fungus, Beauveria bassiana.
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DOI：
10.1016/s0022-2011(02)00045-9
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发表时间：
2002-05
影响因子：
3.4
通讯作者：
C-S Wang;Z.Z. Li;T. Butt
中科院分区：
文献类型：
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作者：
C-S Wang;Z.Z. Li;T. Butt
利用28SrDNA内含子作为分子标记，对球孢白僵菌Bb123和Bb151单孢菌株进行了区分。当共同制定和测定对大蜡螟幼虫这些菌株表现出没有协同增加的毒力，而Bb123通常占主导地位。这项研究表明，任何菌株感染Galleria的成功取决于接种的剂量和方法（注射与浸泡）。在固体培养物上生长的共配制菌株的结果也表明，通常一种菌株占优势，即，在体内和体外都可能发生应变位移。一个菌株在PDA上连续传代培养后被取代的速度部分取决于Bb151和Bb123的比例。共配制的接种物可以拓宽亲本菌株在不同水分活度培养基上具有活性的窗口。共感染确实导致了Galleria宿主内的异核现象。分子生物学研究也表明，异核体是不稳定的，可以恢复到亲本菌株。
A 28S rDNA intron was used as a molecular marker to distinguish between two single spore strains of Beauveria bassiana, Bb123 and Bb151. When co-formulated and assayed against larvae of Galleria mellonella these strains exhibited no synergistic increase in virulence, rather Bb123 usually dominated. This study shows that the success of any strain to infect Galleria is dependent on the dose and method of inoculation (injection versus immersion). The result of co-formulated strains grown on solid culture also showed that usually one strain dominated, i.e., strain displacement could happen both in vivo and in vitro. The speed by which one strain was displaced following successive sub-culturing on PDA partly depended on the ratio of Bb151 and Bb123. The co-formulated inoculum could widen the window over which parent strains would be active on different water activity media. Co-infection did result in heterokaryosis within the Galleria host. Molecular studies also showed that the heterokaryon was not stable and could revert back to the parent strain.