Maternal undernutrition during early pregnancy inhibits postnatal growth of the tibia in the female offspring of rats by alteration of chondrogenesis
Maternal undernutrition during early pregnancy inhibits postnatal growth of the tibia in the female offspring of rats by alteration of chondrogenesis
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DOI：
10.1016/j.ygcen.2017.12.008
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发表时间：
2018-05-01
影响因子：
2.7
通讯作者：
Udagawa, Jun
中科院分区：
文献类型：
--
作者：
Kimura, Tomoko;Hino, Kodai;Udagawa, Jun
流行病学研究表明，出生体重与成年腿长相关。然而，产前营养不良（UN）与产后腿部生长之间的关系仍然存在争议。我们研究了妊娠早期UN对出生后后肢生长的影响，并确定早期胚胎营养不良是否影响大鼠出生后软骨细胞的功能。从妊娠第5.5-11.5天，营养不良的Wistar母鼠以对照组大鼠每日摄入量的40%喂养，并在出生时和16周龄时对对照组和16周龄的股骨、胫骨、躯干或脊柱进行形态学测量。两性的后代都营养不良。我们评估了来自雌性后代新生儿胫骨的培养软骨细胞的细胞增殖和分化，并测定了新生儿骨骺和胚胎肢芽中软骨细胞相关基因的表达水平。与对照大鼠相比，营养不良雌性（而非雄性）后代的胫骨长度在出生时较长，在 16 周龄时较短 (p < .05)。在软骨细胞培养研究中，营养不良的雌性后代中 IGF-1 对细胞增殖的刺激作用 (p < .01) 显着降低，II 型胶原蛋白水平较低 (p < .05)。这些现反式激活剂。 (C) 2017 Elsevier Inc. 保留所有权利。
Epidemiological research has suggested that birth weights are correlated with adult leg lengths. However, the relationship between prenatal undernutrition (UN) and postnatal leg growth remains controversial. We investigated the effects of UN during early pregnancy on postnatal hindlimb growth and determined whether early embryonic malnutrition affects the functions of postnatal chondrocytes in rats.Undernourished Wistar dams were fed 40% of the daily intake of rats in the control groups from gestational days 5.5-11.5, and femurs, tibias, and trunks or spinal columns were morphologically measured at birth and at 16 weeks of age in control and undernourished offspring of both sexes. We evaluated cell proliferation and differentiation of cultured chondrocytes derived from neonatal tibias of female offspring and determined chondrocyte-related gene expression levels in neonatal epiphysis and embryonic limb buds.Tibial lengths of undernourished female, but not male, offspring were longer at birth and shorter at 16 weeks of age (p < .05) compared with those of control rats. In chondrocyte culture studies, stimulating effects of IGF-1 on cell proliferation (p < .01) were significantly decreased and levels of type II collagen were lower in female undernourished offspring (p < .05). These phenomena were accompanied by decreased expression levels of Col2a1 and Igf1r and increased expression levels of Fgfr3 (p < .05), which might be attributable to the decreased expression of specificity protein 1 (p < .05), a key transactivator of Col2a1 and Igf1r.In conclusion, UN stress during early pregnancy reduces postnatal tibial growth in female offspring by altering the function of chondrocytes, likely reflecting altered expression of gene transactivators. (C) 2017 Elsevier Inc. All rights reserved.