Primary Culture of Mesodermal and Endodermal Cells of the Starfish Embryo
Primary Culture of Mesodermal and Endodermal Cells of the Starfish Embryo
复制标题
海星胚胎中胚层和内胚层细胞的原代培养
DOI：
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
M. Dan
中科院分区：
文献类型：
--
作者：
H. Kaneko;Y. Kawahara;M. Dan
摘要介绍了一种海盘车胚胎细胞的培养方法。用浓度为一半的不含Ca 2+、Mg 2+的Jamarin（人工海水）中的0.6 M甘氨酸处理处于间充质迁移阶段的胚胎解离细胞24小时，然后培养24小时，即可获得纯由间充质细胞组成的网络。当处理时间缩短至12小时时，上皮细胞聚集体与网络共存。培养3天后，这些聚集体形成由四种类型的细胞构成的单层片，其可以在形态学上和通过8-苯胺基-1-萘磺酸（1，8-ANS）染色鉴定。这些细胞类型对应于组成食道、胃、肠和体腔袋的细胞。在床单中未发现外胚层细胞。这些结果表明，三种胚层细胞对0.6M甘氨酸处理的耐受性顺序为：e.中胚层、内胚层和外胚层依次为中胚层、内胚层和外胚层。
Abstract A method is described for culturing embryonic cells of the starfish Asterias amurensis. A network composed purely of mesenchyme cells can be obtained by treating dissociated cells of embryos at the mesenchyme migration stage with 0.6 M glycine in half strength Ca2+, Mg2+ free Jamarin (artificial sea water) for 24 hr and subsequently culturing them for 24 hr. When the treatment period is shortened to 12 hr, aggregates of epithelial cells come to coexist with the network. After 3 days of culture, these aggregates form monolayer sheets constituted of four types of cells which can be identified morphologically and by 8-anilino-1-naphthalenesulfonic acid (1, 8-ANS) stainability. These cell types correspond to cells composing the esophagus, the stomach, the intestine and the coelomic pouch. No ectodermal cells were found in the sheet. These results suggest that there is an order of resistibility to 0.6 M glycine treatment among cells of the three germ layers, i. e. mesoderm, endoderm and ectoderm in descending order.
分离海胆胚胎原代间充质细胞的新方法。
影响因子：
3.7
作者：
Ettensohn,CA;McClay,DR
通讯作者：
McClay,DR
通过激光消融研究海胆原肠胚形成过程中次级间充质细胞的作用。
DOI：
10.1242/dev.103.2.317
发表时间：
1988
期刊：
Development (Cambridge, England)
影响因子：
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作者：
Hardin,J
通讯作者：
Hardin,J