In-vitro and in-vivo degradation studies of freeze gelated porous chitosan composite scaffolds for tissue engineering applications
In-vitro and in-vivo degradation studies of freeze gelated porous chitosan composite scaffolds for tissue engineering applications
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DOI：
10.1016/j.polymdegradstab.2016.11.018
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发表时间：
2017-02-01
影响因子：
5.9
通讯作者：
Rehman, Ihtesham Ur
中科院分区：
文献类型：
--
作者：
Qasim, Saad B.;Husain, Shehriar;Rehman, Ihtesham Ur
几十年来，组织工程方法已经被用于重建和恢复受损组织的功能。壳聚糖（CH）与羟基磷灰石（HA）多孔仿生复合支架用于骨再生也已被广泛研究。这些多孔支架在提供成功的再生方面发挥着关键作用，它作为一个三维模板，用于输送营养物质和代谢物，并去除废物。采用扫描电镜（SEM）观察材料的形态变化，傅立叶变换红外光谱（FTIR）结合光声取样（PAS）分析材料的化学变化，pH分析和降解上清液的紫外维斯光谱等方法，研究多孔冻胶壳聚糖（CH）和CH羟基磷灰石（HA）支架的体外和体内降解速率。SEM结果显示表面形貌发生了显著变化。FTIR-PAS光谱显示指纹区域的变化和糖苷键显示断裂的迹象。降解上清液的pH值和UV维斯光谱表明纯样品中CH键断裂。加入HA的标本具有更好的稳定性。体内植入后进行的组织学切片也显示HA负载样品的细胞浸润和延迟降解特征更大。在植入的30天内，纯CH支架显示出完全的体内生物降解。目前的研究结果表明，在多孔模板中加入羟基磷灰石的优点是增强了硬组织再生。此外，它允许生物活性复合支架的简单和成本有效的制造。(C)2016年6月，作者。由Elsevier Ltd.发布。这是CC BY许可下的开放获取文章。
Tissue engineering approaches have been adapted to reconstruct and restore functionality of impaired tissue for decades. Porous biomimetic composite scaffolds of Chitosan (CH) with hydroxyapatite (HA) for bone regeneration have also been extensively studied in the past. These porous scaffolds play a critical role in providing successful regeneration by acting as a three-dimensional template for delivering nutrients and metabolites and the removal of waste by products. The aim of the current study was to investigate in-vitro and in-vivo degradation rates of porous freeze gelated chitosan (CH) and CH hydroxyapatite scaffolds by scanning electron microscopy (SEM) to observe for morphological changes, Fourier Transform Infrared Spectroscopy (FTIR) in conjunction with photo-acoustic sampling (PAS) accessory for the analysis of chemical changes, pH analysis and UV Vis spectroscopy of degraded supernatant. SEM results showed significant alterations in the surface morphology. FTIR-PAS spectra showed changes in the finger print region and glycosidic bonds showed signs of breakage. pH values and UV Vis spectroscopy of the degraded supernatant were indicative of CH bonds scission in neat samples. HA incorporated specimens showed more stability. Histological sections performed after in-vivo implantation also showed greater cellular infiltration and delayed degradation profiles by HA loaded samples. Within 30 days of implantation, neat CH scaffolds showed complete in-vivo biodegradation. The current findings show the advantage of adding hydroxyapatite to porous templates which enhances hard tissue regeneration. In addition, it allows easy and cost effective fabrication of bioactive composite scaffolds. (C) 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license.