Colonic expression of MUC2, MUC5AC, and TFF1 in inflammatory bowel disease in children
Colonic expression of MUC2, MUC5AC, and TFF1 in inflammatory bowel disease in children
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DOI：
10.1097/00005176-200405000-00006
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发表时间：
2004-05-01
影响因子：
2.9
通讯作者：
Marcon, MA
中科院分区：
文献类型：
--
作者：
Shaoul, R;Okada, Y;Marcon, MA
背景资料：炎症性肠病（IBD）中粘蛋白的数量和质量受到影响，这是因为杯状细胞数量减少和每个寡糖侧链糖残基数量减少。粘蛋白类型的改变和异常位置可能通过影响粘液屏障功能而导致潜在病理学，或者可能是对炎症的反应。作者使用过碘酸-希夫/阿尔新蓝染色来区分中性和酸性粘蛋白，并使用成熟杯状细胞粘蛋白MUC 2、MUC 2核心抗原、小凹细胞粘蛋白MUC 5AC和胃三叶因子（TFF 1）的特异性抗体来表征IBD患者结肠组织切片中它们的存在和分布。核心和成熟MUC 2均在来自溃疡性结肠炎（UC）和克罗恩病患者以及来自健康对照的所有结肠杯状细胞中表达。MUC 5AC和TFF 1，这是不正常的结肠组织表达，也表达在散在杯状细胞，共表达MUC 2。在杯状细胞缺失的区域，MUC 2存在于扁平、立方、非杯状细胞样表面细胞的胞质颗粒中。MUC 2核心抗体的染色更强烈且更均匀，表明表达相对不成熟的粘蛋白。这些细胞中的一些也共表达MUC 5AC，但程度较低。这些发现并不是IBD所独有的，但也发现在其他类型的肠道inflammation.Conclusion：这项研究证实了早期的观察，MUC 2是主要的结肠粘蛋白在IBD。它以两种形式出现：杯状细胞中的成熟MUC 2和未成熟MUC 2，特别是在表型上不是杯状细胞的细胞的分泌颗粒中。杯状细胞中的MUC 5AC和TFF 1表达在IBD和结肠的其他炎性病症中是常见的。这些变化可能代表结肠细胞的非特异性修复功能，以补偿屏障功能的损伤。(C)2004年利平科特威廉姆斯威尔金斯。
Background: The quantity and quality of mucins are affected in inflammatory bowel disease (IBD) both because of a reduction in the number of goblet cells and a decrease in the number of sugar residues per oligosaccharide side chain. Alteration in the types of mucins and aberrant location may contribute to the underlying pathology by affecting the mucus barrier function or may instead be a response to inflammation. The authors used the periodic acid-Schiff/Alcian blue stain to distinguish neutral and acidic mucins, and used specific antibodies to the mature goblet cell mucin MUC2, MUC2 core antigen, foveolar cell mucin MUC5AC, and gastric trefoil factor (TFF1), to characterize their presence and distribution in colonic tissue sections from patients with IBD.Results: Both core and mature MUC2 were expressed in all colonic goblet cells from patients with ulcerative colitis (UC) and Crohn disease and from healthy controls. MUC5AC and TFF1, which are not normally expressed by colonic tissue, also were expressed in scattered goblet cells, coexpressing with MUC2. In areas of goblet cell depletion, MUC2 was present in cytoplasmic granules of flattened, cuboidal, nongoblet-cell-like surface cells. The staining was more intense and homogenous with the MUC2 core antibody, suggesting expression of relatively immature mucin. Some of these cells also coexpressed MUC5AC but to a lesser extent. These findings are not unique to IBD but were also found in other types of intestinal inflammation.Conclusion: The study confirms earlier observations that MUC2 is the major colonic mucin in IBD. It appears in two forms: mature MUC2 in goblet cells and immature MUC2 especially in secretory granules of cells that are not phenotypically goblet cells. MUC5AC and TFF1 expression in goblet cells is common in IBD and other inflammatory conditions of the colon. These changes may represent a nonspecific repair function of the colon cells to compensate for damage to barrier function. (C) 2004 Lippincott Williams Wilkins.