Efficient band-selective homonuclear CO-CA cross-polarization in protonated proteins
Efficient band-selective homonuclear CO-CA cross-polarization in protonated proteins
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DOI：
10.1007/s10858-013-9767-1
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发表时间：
2013-08-01
影响因子：
2.7
通讯作者：
Lange, Adam
中科院分区：
文献类型：
--
作者：
Chevelkov, Veniamin;Shi, Chaowei;Lange, Adam
在此之前，我们介绍了一种基于偶极同核交叉极化的带选择性的CO和CA自旋之间的带选择性磁化转移。当CO和CA共振上的有效射频场之和等于自旋速度的两倍时，实现了稳健和有效的再耦合，在质子化泛素中产生了高达33%的磁化转移效率。当CO和CA的各向同性化学位移差大大超过旋转速度时，该方法适用于中等幻角旋转速度和高外加磁场。该方法已在N(I)CO(i-1)CA(i-1)和CA(I)(N-i)CO(i-1)CA(i-1)二维残差相关实验中实现，实现了对泛素的快速有效的固体核磁共振光谱共振归属。
Previously introduced for highly deuterated proteins, band-selective magnetization transfer between CO and CA spins by dipolar-based homonuclear cross polarization is applied here to a protonated protein. Robust and efficient recoupling is achieved when the sum of effective radio-frequency fields on CO and CA resonances equals two times the spinning rate, yielding up to 33 % of magnetization transfer efficiency in protonated ubiquitin. The approach is designed for moderate magic-angle spinning rates and high external magnetic fields when the isotropic chemical shift difference of CO and CA considerably exceeds the spinning rate. This method has been implemented in N(i)CO(i-1)CA(i-1) and CA(i)(N-i)CO(i-1)CA(i-1) two-dimensional interresidual correlation experiments for fast and efficient resonance assignment of ubiquitin by solid-state NMR spectroscopy.