Progressive growth of infantile cutaneous hemangiomas is directly correlated with hyperplasia and angiogenesis of adjacent epidermis and inversely correlated with expression of the endogenous angiogenesis inhibitor, IFN-beta.
Progressive growth of infantile cutaneous hemangiomas is directly correlated with hyperplasia and angiogenesis of adjacent epidermis and inversely correlated with expression of the endogenous angiogenesis inhibitor, IFN-beta.
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DOI：
10.3892/ijo.14.3.401
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发表时间：
1999-03
影响因子：
5.2
通讯作者：
D. Bielenberg;C. Bucana;R. Sanchez;J. Mulliken;J. Folkman;I. Fidler
中科院分区：
文献类型：
--
作者：
D. Bielenberg;C. Bucana;R. Sanchez;J. Mulliken;J. Folkman;I. Fidler
皮肤婴儿血管瘤的进展，通过增殖和退化阶段。由于干扰素治疗，血管生成的负调节，加速退化阶段，我们假设，皮肤婴儿血管瘤与内源性血管生成的正和负调节之间的不平衡。我们检查了30例皮肤血管瘤标本[增殖期（n=15），消退期（n=8），和消退期（n=7）]和对照人皮肤（n=17），固定在福尔马林和石蜡包埋。常规组织学，免疫组织化学，和mRNA原位杂交技术被用来测量表达的阳性血管生成分子碱性成纤维细胞生长因子（bFGF）和血管内皮生长因子/血管通透性因子（VEGF/VPF），和内源性抑制剂的血管生成，干扰素-β（IFN-β）。增殖期血管瘤表达高水平的bFGF和VEGF/VPF，但不表达IFN-β（mRNA和蛋白质）。表皮直接覆盖增生的血管瘤是增生的，含有大量的分裂细胞，表达bFGF和VEGF/VPF，但不表达IFN-β。正常人的表皮和直接覆盖在消退的肿瘤上或远离增殖性血管瘤的表皮不增生，bFGF、VEGF/VPF和IFN-β表达水平正常。这些数据表明，皮肤血管瘤和邻近表皮的增殖与肿瘤和邻近正常组织表达的阳性和阴性血管生成因子之间的不平衡有关。
Cutaneous infantile hemangioma progresses through proliferation and involution phases. Since treatment with interferon, a negative regulator of angiogenesis, accelerates the involution phase, we hypothesized that cutaneous infantile hemangioma is associated with an imbalance between endogenous positive and negative regulators of angiogenesis. We examined 30 specimens of cutaneous hemangioma [proliferative phase (n=15), involuting phase (n=8), and involuted phase (n=7)] and control human skin (n=17), fixed in formalin and embedded in paraffin. Routine histology, immunohistochemistry, and an mRNA in situ hybridization technique were used to measure expression of the positive angiogenic molecules basic fibroblast growth factor (bFGF) and vascular endothelial growth factor/vascular permeability factor (VEGF/VPF), and an endogenous inhibitor of angiogenesis, interferon-beta (IFN-beta). Proliferative phase hemangiomas expressed high levels of bFGF and VEGF/VPF but not IFN-beta (mRNA and protein). The epidermis directly overlying proliferating hemangiomas was hyperplastic, contained numerous dividing cells, and expressed bFGF and VEGF/VPF but not IFN-beta. Epidermis from normal individuals and epidermis directly overlying involuted tumors or at sites distant to the proliferating hemangioma was not hyperplastic and expressed normal levels of bFGF, VEGF/VPF, and IFN-beta. These data suggest that the proliferation of cutaneous hemangiomas and adjacent epidermis is associated with an imbalance between positive and negative angiogenic factors expressed by the neoplasm and adjacent normal tissue.