Yeast as a model system for studying endocytosis.
Yeast as a model system for studying endocytosis.
复制标题
DOI：
10.1006/excr.2001.5373
复制
发表时间：
2001-11
影响因子：
3.7
通讯作者：
J. Shaw;K. B. Cummings;G. Huyer;S. Michaelis;B. Wendland
中科院分区：
文献类型：
--
作者：
J. Shaw;K. B. Cummings;G. Huyer;S. Michaelis;B. Wendland
内吞作用是膜运输过程，通过该过程，质膜组分和细胞外物质内化到细胞质囊泡中并递送到早期和晚期内体，最终再循环回到质膜或到达溶酶体/液泡。芽殖酵母酿酒酵母已被证明是一个宝贵的系统，用于识别参与内吞作用的蛋白质，并阐明内在化和后内在化事件的机制。通过在酵母中的遗传研究和在哺乳动物细胞中的生物化学研究，已经变得明显的是，多个细胞过程与内吞作用有关，包括肌动蛋白细胞骨架动力学、泛素化、脂质修饰和信号转导。在这篇综述中，我们将重点介绍酵母胞吞作用领域最令人兴奋的最新发现。具体来说，我们将解决的肌动蛋白细胞骨架的参与内化，泛素化的作用，作为一个调节器的多个步骤的内吞在酵母中，和内吞蛋白的分选到回收和空泡途径。
Endocytosis is the membrane trafficking process by which plasma membrane components and extracellular material are internalized into cytoplasmic vesicles and delivered to early and late endosomes, eventually either recycling back to the plasma membrane or arriving at the lysosome/vacuole. The budding yeast Saccharomyces cerevisiae has proven to be an invaluable system for identifying proteins involved in endocytosis and elucidating the mechanisms underlying internalization and postinternalization events. Through genetic studies in yeast and biochemical studies in mammalian cells, it has become apparent that multiple cellular processes are linked to endocytosis, including actin cytoskeletal dynamics, ubiquitylation, lipid modification, and signal transduction. In this review, we will highlight the most exciting recent findings in the field of yeast endocytosis. Specifically, we will address the involvement of the actin cytoskeleton in internalization, the role of ubiquitylation as a regulator of multiple steps of endocytosis in yeast, and the sorting of endocytosed proteins into the recycling and vacuolar pathways.