A modified approach for programmed electrical stimulation in mice: Inducibility of ventricular arrhythmias
A modified approach for programmed electrical stimulation in mice: Inducibility of ventricular arrhythmias
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DOI：
10.1371/journal.pone.0201910
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发表时间：
2018-08-22
期刊：
影响因子：
3.7
通讯作者：
Makimoto, Hisaki
中科院分区：
文献类型：
--
作者：
Clasen, Lukas;Eickholt, Christian;Makimoto, Hisaki
背景小鼠是基础心血管研究领域的主要模式生物，目的探讨一种改良的程序电刺激治疗室性心律失常（VA）的方法方法对检索PubMed数据库的关于小鼠中PES的当前方法的文献进行系统综述，结果显示VA诱导率低，范围很广（4.6 +/- 10.7%）。基于该文献综述，开发了具有3至10次额外刺激的改良PES方案，并在麻醉的野生型小鼠（C57 BL/6 J，n = 12）中与使用多达3次额外刺激的常规PES方案进行了比较。诱导VA，分类根据兰贝斯公约，进行了评估，建立心律失常评分以及一个新的心律失常评分的基础上VA daily.ResultsPES与修改后的方法提出的发生率和VA的持续时间相比，传统的PES（0%与50%;新的VA评分P = 0.0002）。特别是，偶联>6次额外刺激可提高VA的诱导。主要为室性早搏（n = 6）和室性心动过速
BackgroundElectrophysiological studies in mice, the prevailing model organism in the field of basic cardiovascular research, are impeded by the low yield of programmed electrical stimulation (PES).ObjectiveTo investigate a modified approach for ventricular arrhythmia (VA) induction and a novel scoring system in mice.MethodA systematic review of literature on current methods for PES in mice searching the PubMed database revealed that VA inducibility was low and ranged widely (4.6 +/- 10.7%). Based on this literature review, a modified PES protocol with 3 to 10 extrastimuli was developed and tested in comparison to the conventional PES protocol using up to 3 extrastimuli in anesthetized wildtype mice (C57BL/6J, n = 12). Induced VA, classified according to the Lambeth Convention, were assessed by established arrhythmia scores as well as a novel arrhythmia score based on VA duration.ResultsPES with the modified approach raised both the occurrence and the duration of VA compared to conventional PES (0% vs 50%; novel VA score p = 0.0002). Particularly, coupling of >6 extrastimuli raised the induction of VA. Predominantly, premature ventricular complexes (n = 6) and ventricular tachycardia