Evaluation of immobilized boronates for studies of adenine and pyridine nucleotide metabolism.
Evaluation of immobilized boronates for studies of adenine and pyridine nucleotide metabolism.
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评估固定化硼酸盐在腺嘌呤和吡啶核苷酸代谢研究中的应用。
DOI：
10.1016/0003-2697(83)90732-7
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发表时间：
1983
影响因子：
2.9
通讯作者：
Jacobson,MK
中科院分区：
文献类型：
--
作者：
Alvarez-Gonzalez,R;Juarez-Salinas,H;Jacobson,EL;Jacobson,MK
比较了三种不同的固定化硼酸盐在腺嘌呤和吡啶核苷酸代谢研究中的可能用途。其中包括聚丙烯酰胺、琼脂糖凝胶和阳离子交换树脂 Bio-Rex 70 的硼酸酯衍生物。比较了相对结合亲和力、结合能力和洗脱特性。在所使用的条件下，Sepharose 和 Bio-Rex 70 衍生物选择性保留含有两组或多组 1,2 顺式二醇基团的核苷酸。由于大部分细胞核酸仅含有一组或更少的 1,2 顺式二醇基团，因此这些硼酸酯衍生物对于分离酸溶性核苷酸、NAD 和二腺苷 5',54-四磷酸以及酸不溶性聚合物聚（ADP-核糖）非常有用。 Bio-Rex 70 衍生物具有结合选择性、容量和洗脱特性的特别有用的组合。介绍了该树脂在 NAD 和聚（ADP-核糖）代谢研究中的具体应用。
Three different immobilized boronates were compared with respect to their possible utility in studies of adenine and pyridine nucleotide metabolism. These included boronate derivatives of polyacrylamide, Sepharose, and the cation-exchange resin, Bio-Rex 70. The relative binding affinities, binding capacities, and elution properties were compared. Under the conditions utilized, the Sepharose and Bio-Rex 70 derivatives selectively retained nucleotides containing two or more sets of 1,2 cis-diol groups. Since the bulk of cellular nucleic acids contain but a single set of 1,2 cis-diol groups or less, these boronate derivatives are very useful for the isolation of the acid-soluble nucleotides, NAD and diadenosine 5′,5‴-tetraphosphate, and for the acid-insoluble polymer, poly(ADP-ribose). The Bio-Rex 70 derivative had a particularly useful combination of binding selectivity, capacity, and elution characteristics. Specific applications of this resin for studies of NAD and poly(ADP-ribose) metabolism are presented.