Viral persistence in colorectal cancer cells infected by Newcastle disease virus.
Viral persistence in colorectal cancer cells infected by Newcastle disease virus.
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DOI：
10.1186/1743-422x-11-91
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发表时间：
2014-05-16
期刊：
影响因子：
4.8
通讯作者：
Shafee N
中科院分区：
文献类型：
--
作者：
Chia SL;Yusoff K;Shafee N
新城疫病毒(NDV)是副粘病毒科新城疫病毒属单链RNA病毒，是治疗癌症的候选病毒制剂。在临床研究中观察到了有希望的反应。尽管这种病毒具有很高的潜力，但它在癌细胞中发展为持续性感染的可能性尚未得到调查。新城疫病毒在癌细胞中的持续感染可能会使细胞对病毒的杀伤不那么敏感。这将产生一批仍能存活并对治疗产生抗药性的癌细胞。在一系列结直肠癌细胞株的感染实验中，我们意外地观察到新城疫病毒在SW480细胞中持续感染的发展，而在其他被测试的细胞株中没有。该细胞群命名为SW480P，在再感染实验中显示出对新城疫病毒杀伤的抵抗力。SW480P细胞保留了新城疫病毒基因组，并产生了病毒后代，但空斑形成能力降低。这些观察表明，新城疫病毒可以在癌细胞中持续感染，在临床环境中使用新城疫病毒时需要考虑这一因素。
Newcastle disease virus (NDV), a single-stranded RNA virus of the family Paramyxoviridae, is a candidate virotherapy agent in cancer treatment. Promising responses were observed in clinical studies. Despite its high potential, the possibility of the virus to develop a persistent form of infection in cancer cells has not been investigated. Occurrence of persistent infection by NDV in cancer cells may cause the cells to be less susceptible to the virus killing. This would give rise to a population of cancer cells that remains viable and resistant to treatment. During infection experiment in a series of colorectal cancer cell lines, we adventitiously observed a development of persistent infection by NDV in SW480 cells, but not in other cell lines tested. This cell population, designated as SW480P, showed resistancy towards NDV killing in a re-infection experiment. The SW480P cells retained NDV genome and produced virus progeny with reduced plaque forming ability. These observations showed that NDV could develop persistent infection in cancer cells and this factor needs to be taken into consideration when using NDV in clinical settings.