Dendritic calcium spikes are tunable triggers of cannabinoid release and short-term synaptic plasticity in cerebellar Purkinje neurons.
Dendritic calcium spikes are tunable triggers of cannabinoid release and short-term synaptic plasticity in cerebellar Purkinje neurons.
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DOI：
10.1523/jneurosci.5284-05.2006
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发表时间：
2006-05-17
期刊：
影响因子：
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通讯作者：
Häusser M
中科院分区：
文献类型：
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作者：
Rancz EA;Häusser M
了解树突兴奋性和突触可塑性之间的关系对于确定树突如何调节神经元的输入-输出功能至关重要。树突状钙峰与突触效能的长期变化的诱导有关。在这里，我们使用小脑浦肯野细胞树突的直接记录显示，突触激活的局部树突状钙尖峰是大麻素释放的有力触发器，在平行纤维突触突触的突触功效产生深刻的和短期的减少。通过调节树突状BK型钙激活钾通道增强树突状兴奋性改善了树突状钙峰的传播，并以空间特异性为代价增强了大麻素的释放。我们的研究结果表明，树突状细胞的钙峰提供了一个本地和可调的重合检测机制，重新调整突触增益时，同步活动达到阈值，并揭示了树突状细胞的兴奋性和诱导突触可塑性的调节之间的紧密联系。
Understanding the relationship between dendritic excitability and synaptic plasticity is vital for determining how dendrites regulate the input-output function of the neuron. Dendritic calcium spikes have been associated with the induction of long-term changes in synaptic efficacy. Here we use direct recordings from cerebellar Purkinje cell dendrites to show that synaptically activated local dendritic calcium spikes are potent triggers of cannabinoid release, producing a profound and short-term reduction in synaptic efficacy at parallel fiber synapses. Enhancing dendritic excitability by modulating dendritic BK-type calcium-activated potassium channels improves the spread of dendritic calcium spikes and enhances cannabinoid release at the expense of spatial specificity. Our findings reveal that dendritic calcium spikes provide a local and tunable coincidence detection mechanism that readjusts synaptic gain when synchronous activity reaches a threshold, and reveal a tight link between regulation of dendritic excitability and the induction of synaptic plasticity.