Gap junctional communication compartments in the Drosophila wing disk.
Gap junctional communication compartments in the Drosophila wing disk.
复制标题
果蝇翼盘中的间隙连接通讯室。
DOI：
10.1073/pnas.79.10.3232
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发表时间：
1982
影响因子：
11.1
通讯作者：
Lo,CW
中科院分区：
文献类型：
--
作者：
Weir,MP;Lo,CW
我们用荧光染料荧光黄细胞内注射的方法研究了果蝇翅膀成像盘细胞的缝隙连接通讯特性。荧光黄的细胞间通道被限制在将翼盘一分为二的边界线上。我们把每一半称为“通讯隔间”，因为隔间内有高水平的缝隙连接交换，而隔间之间的交换要低得多。将各室的位置与发育命运图进行比较，结果表明，在成人背侧间胸的前半部，一个交通室的细胞形成角质结构，而另一个交通室的细胞形成后部结构。这条通讯限制线似乎与细胞谱系研究中观察到的前后部发育间隔之间的线一致。我们认为缝隙连接通信限制可能在发育间隔的产生或维持中起着普遍的作用。
We have examined the gap junctional communication properties of cells in the wing imaginal disk of Drosophila, using intracellular injection of the fluorescent dye tracer Lucifer Yellow. The cell-to-cell passage of Lucifer Yellow is restricted at a boundary line that divides the wing disk into halves. We refer to each half as a "communication compartment" because there is a high level of gap junctional exchange within a compartment and much lower exchange between compartments. Comparison of the positions of the compartments with developmental fate maps suggests that cells in one communication compartment give rise to cuticular structures in the anterior half of the adult dorsal mesothorax, and cells in the other compartment contribute to posterior structures. This communication-restriction line appears to be coincident with the line between the anterior and posterior developmental compartments observed in studies of cell lineage. We propose that gap junctional communication restrictions may play a general role in generating or maintaining developmental compartments.