Human P3(450): cDNA and complete amino acid sequence.
Human P3(450): cDNA and complete amino acid sequence.
复制标题
人 P3(450)：cDNA 和完整氨基酸序列。
作者：
A. Jaiswal;D. Nebert;F. Gonzalez
从人肝脏Xgt11 cDNA文库中，用双脱氧法对最长的oDNA插入片段进行测序。完整蛋白（Mr = 58,294）有515个残基。起始密码子、酶活性位点半胱氨酸的oodon和假定的聚腺苷化信号在方框中显示。由于Alu重复序列的4个拷贝，人类p3i50mrna （3.3 kb）异常长。同源小鼠P3I50(1)和大鼠P450d(2)基因已经测序，在它们的3'非翻译区不包含这样的Alu序列。
From a Xgt11 cDNA library derived from human liver, the longest oDNA insert was sequenced by the dideoxy method. The complete protein (Mr = 58,294) has 515 residues. The initiation codon, oodon for the cysteine in the enzyme active-site, and putative polyadenylation signal are shown in boxes. The human P3I5O mRNA (3.3 kb) is unusually long, due to four copies of Alu repetitive sequences. The orthologous mouse P3I50 (1) and rat P450d (2) genes have already been sequenced and contain no such Alu sequences in their 3' nontranslated regions.