Neisserial antigen H.8 is immunogenic in patients with disseminated gonococcal and meningococcal infections.
Neisserial antigen H.8 is immunogenic in patients with disseminated gonococcal and meningococcal infections.
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奈瑟菌抗原 H.8 在播散性淋球菌和脑膜炎球菌感染患者中具有免疫原性。
DOI：
10.1093/infdis/151.4.650
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Cannon,JG
中科院分区：
文献类型：
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作者：
Black,JR;Black,WJ;Cannon,JG
淋病奈瑟菌和脑膜炎奈瑟菌菌株之间和菌株内部的抗原多样性使这些菌株的发病机制研究变得复杂，并阻碍了疫苗的开发。我们之前描述了致病性奈瑟菌常见的独特表面抗原 (H.8)。现在，我们通过检测 13 名播散性奈瑟氏球菌感染患者对该抗原的抗体反应，证明了 H.8 抗原的体内表达。酶联免疫吸附试验中的结合抑制研究表明，来自康复期患者的每一份血清样本都会阻断感染性脑膜炎球菌或淋球菌菌株与针对 H.8 抗原的单克隆抗体之间的结合 (P< .005)。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质印迹进行的测试表明，每个恢复期血清中对与 H.8 抗原共同迁移的抗原有 IgG 反应。通过探测表达 H.8 抗原的重组噬菌体证实了该抗体反应的特异性。 H.8 抗原的共性和免疫原性表明它在淋球菌和脑膜炎球菌疾病的发病机制中可能发挥作用，并且具有作为淋球菌和脑膜炎球菌疾病疫苗成分的潜力。
Antigenic diversity among and within strains ofNeisseria gonorrhoeaeandNeisseria meningitidishas complicated studies of the pathogenesis of these strains and obstructed vaccine development. We previously described a distinct surface antigen (H.8) common to pathogenicNeisseria. We have now demonstrated in vivo expression of the H.8 antigen by detecting antibody responses to the antigen in 13 patients with disseminated neisserial infections. Each serum sample from a convalescent patient blocked the binding between the infecting meningococcal or gonococcal strain and a monoclonal antibody directed to the H.8 antigen, as demonstrated by binding-inhibition studies in enzyme-linked immunosorbent assays (P< .005). Testing by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting demonstrated an IgG response in each convalescent serum to an antigen co-migrating with the H.8 antigen. Specificity of this antibody response was confirmed by probing recombinant bacteriophage that expressed the H.8 antigen. The commonality and the immunogenicity of the H.8 antigen indicate its possible role in the pathogenesis of, and its potential as a vaccine component for, gonococcal and meningococcal diseases.