Genome-wide analysis of eukaryotic twin CX9C proteins
Genome-wide analysis of eukaryotic twin CX9C proteins
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DOI：
10.1039/c0mb00058b
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发表时间：
2010-01-01
影响因子：
--
通讯作者：
Cavallaro, Gabriele
中科院分区：
文献类型：
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作者：
Cavallaro, Gabriele
双CX 9 C蛋白是真核生物蛋白，其名称来源于其特征基序，由两对半胱氨酸组成，形成两个二硫键，稳定卷曲螺旋-螺旋-卷曲螺旋-螺旋（CHCH）折叠。这些蛋白质中最具特征的是Cox 17，一种在细胞色素c氧化酶生物发生中起作用的铜伴侣，以及Mia 40，一种用于蛋白质输入到线粒体膜间空间（IMS）的系统的中心组分。然而，这些蛋白质的可能功能范围尚不清楚。本文采用生物信息学方法和文献分析相结合的方法，对真核生物中的孪生CX 9 C蛋白进行了系统的检索，并将其归类为可能的同源物。我们的研究结果表明，大多数双胞胎CX 9 C蛋白的功能围绕着共同的主题而变化，即发挥支架作用，这可以将它们在线粒体结构和功能中的作用联系起来。这项研究将加强目前的真核生物蛋白质组的注释，并将提供一个合理的基础，为未来的实验工作，旨在更深入地了解这类显着的蛋白质。
Twin CX9C proteins are eukaryotic proteins that derive their name from their characteristic motif, consisting of two pairs of cysteines that form two disulfide bonds stabilizing a coiled coil-helix-coiled coil-helix (CHCH) fold. The best characterized of these proteins are Cox17, a copper chaperone acting in cytochrome c oxidase biogenesis, and Mia40, the central component of a system for protein import into the mitochondrial inter-membrane space (IMS). However, the range of possible functions for these proteins is unclear. Here, we performed a systematic search of twin CX9C proteins in eukaryotic organisms, and classified them into groups of putative homologues, by combining bioinformatics methods with literature analysis. Our results suggest that the functions of most twin CX9C proteins vary around the common theme of playing a scaffolding role, which can tie their observed roles in mitochondrial structure and function. This study will enhance the present annotation of eukaryotic proteomes, and will provide a rational basis for future experimental work aimed at a deeper understanding of this remarkable class of proteins.