Microsphere nanoscopy for imaging of actin proteins
Microsphere nanoscopy for imaging of actin proteins
复制标题
用于肌动蛋白成像的微球纳米镜
DOI：
--
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
V. Astratov
中科院分区：
文献类型：
--
作者：
A. Brettin;K. F. Blanchette;Y. Nesmelov;N. Limberopoulos;A. Urbas;V. Astratov
接触微透镜的使用首先开始被用作一种简单的方法，该方法允许使用可见光解析纳米尺寸的物体。已经报道了纳米等离子体结构的分辨率超过经典衍射极限。然而，该机制尚未完全理解，无论是从光子纳米射流，等离子体激元极化激元，局部表面等离子体激元共振，金属物体中的相干模式激发，或在微球透镜本身的光学共振仍然是有争议的文献。在这项工作中，我们采用接触高指数微球的荧光成像肌动蛋白丝。将微球嵌入弹性聚合物板中。事实上，细丝具有纳米级的宽度，比衍射极限小得多，大大简化了分辨率分析。使用有限数值孔径（NA=0.6）的显微镜物镜，通过微球的蛋白质分辨率为λ/1.5，在没有微球的情况下分辨率为λ/0.5。
The use of contact microlenses first started being used as a simple method which allows resolving nanometer sized objects using visible light. The resolution beyond the classical diffraction limit has been reported for nanoplasmonic structures. However the mechanism is not fully understood whether it is from photonic nanojets, plasmon-polaritons, localized surface plasmon resonances, coherent modal excitation in metallic objects, or optical resonances in the microspherical lens itself is still debated in the literature. In this work, we applied contact high-index microspheres for fluorescence imaging of actin protein filaments. The microspheres were embedded in elastopolymer slabs. The fact that the filaments have nanometer-scale width, much smaller than the diffraction limit, significantly simplified the resolution analysis. Using microscope objective with a limited numerical aperture (NA=0.6), we achieve a resolution of ∼λ/1.5 for proteins through the microsphere and a resolution of ∼λ/0.5 without a microsphere.