THE RELATIONSHIP OF GLIAL FIBRILLARY ACIDIC PROTEIN TO THE SHAPE, MOTILITY, AND DIFFERENTIATION OF HUMAN ASTROCYTOMA-CELLS
THE RELATIONSHIP OF GLIAL FIBRILLARY ACIDIC PROTEIN TO THE SHAPE, MOTILITY, AND DIFFERENTIATION OF HUMAN ASTROCYTOMA-CELLS
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DOI：
10.1016/0014-4827(82)90328-7
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发表时间：
1982-01-01
影响因子：
3.7
通讯作者：
RAPPORT, MM
中科院分区：
文献类型：
--
作者：
DUFFY, PE;HUANG, YY;RAPPORT, MM
胶质细胞酸性蛋白（GFAP），一种主要局限于[多发性硬化]星形胶质细胞的蛋白质，通过使用延时摄影和免疫过氧化物酶法研究了组织培养中星形细胞瘤细胞的形状、运动性、分化和恶性程度。星形胶质细胞的形态与GFAP的分布有一定的关系。梭形细胞胞体和突起内GFAP丰富。在圆形或多角形细胞中，GFAP主要分布于核周，突起不发达。随着过程的发展，GFAP以密集的平行阵列从细胞核中延伸出来，辐射到正在发展的过程中。完全分化的细胞呈星状，GFAP含量丰富。星形胶质细胞的运动和GFAP之间也观察到的关系。星状细胞，缺乏运动和相对僵硬的姿态的过程中，含有丰富的GFAP往往形成密集的平行阵列延伸到过程中，他们的发展。梭形细胞的延伸和收缩过程和积极的迁移也包含了丰富的GFAP，但没有组织成平行阵列。在突起（生长锥）顶端的球状突起含有丰富的GFAP。星形细胞突起沿着间断性突起内也有丰富的GFAP。与这些观察结果相反，在分化程度较低的细胞中，突起的回缩、高度的可塑性（起伏运动）和多向运动通常与GFAP的缺乏有关。GFAP纤维可能抑制运动的巨大可塑性，但不抑制伸展-收缩运动。在有丝分裂过程中，GFAP在纺锤体和细胞间桥中稀疏。秋水仙胺导致GFAP从细胞的突起和外周部分消失，并集中在细胞核附近。在恶性肿瘤的培养物中，未分化和大的多列细胞通常显示GFAP稀疏，但偶尔可见分化良好的星形或梭形细胞含有丰富的GFAP。相反，虽然来自良性肿瘤的培养物可能具有分散的分化较低的细胞，但具有发育良好的突起的分化细胞染色最密集，并解释了来自这些肿瘤的组织中GFAP的高浓度。
Glial fibrillary acidic protein (GFAP), a protein largely limited to [multiple sclerosis] astrocytes, was studied in relation to the shape, motility, differentiation and malignancy of astrocytoma cells in tissue culture by use of time-lapse photography and the immunoperoxidase method. A relationship was observed between the shape of astrocytes and the distribution of GFAP. Spindle-shaped cells showed abundant GFAP in the cell body and processes. In round or polyhedral cells without well developed processes the GFAP was largely perinuclear. As processes developed, GFAP extended out from the nucleus in dense parallel arrays that radiated into the developing processes. Fully differentiated cells with stellate shape had abundant GFAP throughout. A relationship was also observed between the motility of astrocytes and GFAP. Stellate-shaped cells, showing paucity of locomotion and relatively rigid postures of processes, contained an abundance of GFAP which tended to form dense parallel arrays extending into the processes during their development. Spindle-shaped cells with extending and retracting processes and active migration also contained an abundance of GFAP, but not organized into parallel arrays. Bulbous dilatations at the tips of processes (growth cones) contained abundant GFAP. There was also abundant GFAP in the intermittent dilatations along the processes of stellate cells. In contrast to these observations, a retraction of processes, a high degree of plasticity (undulating motion) and multidirectional locomotion were often associated with a paucity of GFAP in less differentiated cells. GFAP filaments may be inhibitory to great plasticity of motion, but not to extension-retraction movements. During mitosis GFAP was sparse at the spindle and in intercellular bridges. Colcemid caused GFAP to disappear from processes and peripheral parts of the cell and to become concentrated near the nucleus. In cultures derived from malignant tumors, undifferentiated and large multincleated cells usually showed sparsity of GFAP, but occasional well differentiated stellate or spindle-shaped cells containing abundant GFAP were seen. Conversely, although cultures derived from benign tumors may have scattered less well differentiated cells, the differentiated cells with well developed processes were most densely stained and account for the high concentration of GFAP in tissue from these tumors.