Immobilization of Lipase using Alginate Hydrogel Beads and Enzymatic Evaluation in Hydrolysis of p-Nitrophenol Butyrate
Immobilization of Lipase using Alginate Hydrogel Beads and Enzymatic Evaluation in Hydrolysis of p-Nitrophenol Butyrate
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DOI：
10.5012/bkcs.2013.34.9.2741
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发表时间：
2013-09
影响因子：
1.7
通讯作者：
Shuang Zhang;W. Shang;Xiaoxi Yang;Shujuan Zhang;Xiaogang Zhang;Jiawei Chen
中科院分区：
文献类型：
--
作者：
Shuang Zhang;W. Shang;Xiaoxi Yang;Shujuan Zhang;Xiaogang Zhang;Jiawei Chen
酶的固定化是酶研究和产业化的关键问题之一。本文报道了一种以海藻酸钠为载体的南极假丝酵母脂肪酶B（CALB）的简易固定化方法。在钙离子存在下，酶-海藻酸钠悬浮液可在室温下交联形成具有多孔结构的珠粒，酶CALB分散在珠粒中。以对硝基苯酚丁酸酯为底物，在水相中进行酶解反应，验证了酶-海藻酸钠复合物的活性。讨论了温度、pH值、包埋时间、冻干时间等因素对反应的影响。重复使用循环实验表明，酶-藻酸盐复合物的活性保持没有显着的失活高达6个重复循环的水解对硝基苯酚丁酸酯。
The immobilization of enzyme is one of the key issues both in the field of enzymatic research and industrialization. In this work, we reported a facile method to immobilize Candida Antarctica lipase B (CALB) in alginate carrier. In the presence of calcium cation, the enzyme-alginate suspension could be cross-linked to form beads with porous structure at room temperature, and the enzyme CALB was dispersed in the beads. Activity of the enzyme-alginate composite was verified by enzymatic hydrolysis reaction of p-nitrophenol butyrate in aqueous phase. The effects of reaction parameters such as temperature, pH, embedding and lyophilized time on the reactive behavior were discussed. Reuse cycle experiments for the hydrolysis of p-nitrophenol butyrate demonstrated that activity of the enzyme-alginate composite was maintained without marked deactivation up to 6 repeated cycles.