Widespread p53 overexpression in human malignant tumors. An immunohistochemical study using methacarn-fixed, embedded tissue.
Widespread p53 overexpression in human malignant tumors. An immunohistochemical study using methacarn-fixed, embedded tissue.
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发表时间：
1992
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通讯作者：
P. Porter;A. Gown;Steven G. Kramp;M. Coltrera
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作者：
P. Porter;A. Gown;Steven G. Kramp;M. Coltrera
P53是一种核蛋白，通过突变和过度表达，在人类恶性肿瘤的发展过程中发挥着重要作用。作者使用了一种名为1801的单抗，并使用去石蜡切片观察了255例恶性肿瘤和良性肿瘤中P53的过度表达。总体而言，免疫组织化学检测到的P53在39%的恶性肿瘤中过表达，在不同的肿瘤类型中有相当大的差异(34%的乳腺癌，92%的卵巢癌，33%的软组织肉瘤)。均一、异质和局灶性免疫染色模式被注意到。除了极少数例外，没有注意到任何良性肿瘤的免疫染色。在邻近的良性组织或一系列胎儿组织中未见免疫染色。这是首次证明在酒精固定的包埋组织中广泛存在的P53过表达，并证实了P53在人类恶性肿瘤中所起的主要作用。
p53 is a nuclear protein believed to play an important role, through mutation and overexpression, in the progression of human malignant tumors. The authors employed a monoclonal antibody, 1801, and investigated overexpression of p53 in a series of 255 malignant and benign tumors, using deparaffinized sections of methacarn-fixed tissue. Overall, immunohistochemically detected p53 overexpression was found in 39% of malignant tumors, with considerable variation within individual tumor types (34% of breast carcinomas, 92% of ovarian carcinomas, 33% of soft tissue sarcomas). Homogenous, heterogenous, and focal immunostaining patterns were noted. With rare exceptions, no immunostaining of any benign tumors was noted. No immunostaining was found in adjacent, benign tissues, or in a series of fetal tissues. This is the first demonstration of widespread p53 overexpression in alcohol-fixed, embedded tissue and confirms the major role played by p53 in human malignancies.