Repletion with (n-3) fatty acids reverses bone structural deficits in (n-3)-deficient rats
Repletion with (n-3) fatty acids reverses bone structural deficits in (n-3)-deficient rats
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DOI：
10.1093/jn/134.2.388
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发表时间：
2004-02-01
影响因子：
4.2
通讯作者：
Watkins, BA
中科院分区：
文献类型：
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作者：
Reinwald, S;Li, Y;Watkins, BA
(n-3)PUFA缺乏和补充对骨机械性能的影响尚未研究。主要研究目的是评估先前报道的影响骨形成速率的骨组织隔室脂肪酸组成的变化是否会影响骨建模和机械性能。在这项研究中，研究了三组大鼠，第二代（n-3）-缺乏，（n-3）-充满，和对照（n-3）-足够。（n-3）-充足日粮含有α-亚麻酸[LNA，18：3（n-3），占总脂肪酸的2.6%]和二十二碳六烯酸[DHA，22：6（n-3），占总脂肪酸的1.3%]。慢性（n-3）缺乏大鼠后肢组织（骨骼和肌肉）的脂肪酸组成显示（n-6）PUFA显著增加[20：4（n-6）、22：4（n-6）和22：5（n-6）]，（n-3）PUFA相应减少[18：3（n-3）、20：5（n-3）、22：5（n-3）和22：6（n-3）]。骨力学性能（能量峰值负荷）的测量表明，（n-3）的不足降低了结构的完整性。大鼠充满（n-3）脂肪酸表现出加速骨建模（横截面几何形状）和改善的二阶矩在tidry与对照组（n-3）-足够的大鼠28天的饮食治疗后。这项研究表明，补充膳食（n-3）脂肪酸可恢复骨室中（n-6）/（n-3）PUFA的比例，并逆转（n-3）缺乏大鼠受损的骨模型。
(n-3) PUFA deficiency and repletion effects on bone mechanical properties have not been examined. The primary research aim was to evaluate whether changes in the fatty acid composition of bone tissue compartments previously reported to influence bone formation rates would affect bone modeling and mechanical properties. In this investigation, three groups of rats were studied, second generation (n-3)-deficient, (n-3)-repleted, and a control (n-3)-adequate. The (n-3)-adequate diet contained a-linolenic acid [LNA, 18:3(n-3), 2.6% of total fatty acids) and docosahexaenoic acid [DHA, 22:6(n-3),1.3% of total fatty acids]. Fatty acid composition of the hindlimb tissues (bone and muscle) of chronically (n-3)-deficient rats revealed a marked increase in (n-6) PUFA [20:4(n-6), 22:4(n-6), and 22:5(n-6)] and a corresponding decrease in (n-3) PUFA [1 8:3(n-3), 20:5(n-3), 22:5(n-3) and 22:6(n-3)]. Measurement of bone mechanical properties (energy to peak load) of tibiae showed that (n-3) deficiency diminished structural integrity. Rats repleted with (n-3) fatty acids demonstrated accelerated bone modeling (cross-sectional geometry) and an improved second moment in tibiae compared with control (n-3)-adequate rats after 28 d of dietary treatment. This study showed that repletion with dietary (n-3) fatty acids restored the ratio of (n-6)/(n-3) PUFA in bone compartments and reversed compromised bone modeling in (n-3)- deficient rats.