Use of glycopeptidolipid core antigen for serodiagnosis of Mycobacterium avium complex pulmonary disease in immunocompetent patients
Use of glycopeptidolipid core antigen for serodiagnosis of Mycobacterium avium complex pulmonary disease in immunocompetent patients
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DOI：
10.1128/cdli.12.1.44-51.2005
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发表时间：
2005-01-01
期刊：
影响因子：
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通讯作者：
Kobayashi, K
中科院分区：
文献类型：
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作者：
Kitada, S;Maekura, R;Kobayashi, K
我们报告了一种以鸟分枝杆菌复合群（MAC）特异性糖肽脂（GPL）核心为抗原的酶免疫测定（EIA）血清学诊断方法的发展。在这项研究中，我们通过EIA证实了免疫活性MAC病患者血清中存在GPL核心抗体。评价了定量GPL核心抗体的EIA作为肺MAC疾病血清学诊断的临床工具。与MAC定植患者、堪萨斯分枝杆菌病或结核病患者和健康受试者相比，MAC肺病患者血清中GPL核心抗体（免疫球蛋白G、A和M）显著增加。以GPL为核心的EIA诊断MAC肺病的敏感性和特异性分别为IgG 72.6%和92.2%，IgA 92.5%和95.1%，IgM 78.3%和91.0%。通过测量抗GPL核心抗原的免疫球蛋白A抗体获得最佳灵敏度和特异性。GPL核心抗体的水平反映了疾病的活动性，因为它在对化疗有反应的治愈MAC患者中下降。抗GPL核心抗体的血清测定可用于肺MAC疾病的诊断和疾病活动性评估。
We report the development of a serodiagnostic method for Mycobacterium avium complex (MAC) disease with an enzyme immunoassay (EIA) with the MAC-specific glycopeptidolipid (GPL) core as the antigen. In this study, we confirmed by EIA that the GPL core antibody was in the sera of immunocompetent patients with MAC disease. The EIA for quantifying the GPL core antibody was evaluated as a clinical tool for serodiagnosis of pulmonary MAC disease. A significant increase in GPL core antibodies (immunoglobulins G, A, and M) was detected in sera of patients with MAC pulmonary diseases when they were compared to patients who were colonized with MAC, patients with Mycobacterium kansasii disease or tuberculosis, and healthy subjects. The sensitivities and specificities of the GPL core-based EIA for diagnosis of MAC pulmonary disease were 72.6% and 92.2%, respectively, for IgG, 92.5% and 95.1%, respectively, for IgA, and 78.3% and 91.0%, respectively, for IgM. The best sensitivity and specificity were obtained by measuring immunoglobulin A antibodies against GPL core antigen. The level of GPL core antibodies reflected disease activity, since it decreased in cured MAC patients who had responded to chemotherapy. Measurement of serum antibodies against GPL core is useful for both diagnosis and assessment of disease activity in MAC disease of the lung.