The SnRK2-APC/C(TE) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways.
The SnRK2-APC/C(TE) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways.
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SnRK2-APC/C(TE) 调节模块介导赤霉酸和脱落酸途径的拮抗作用。
DOI：
10.1038/ncomms8981
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发表时间：
2015-08-14
影响因子：
16.6
通讯作者：
Wan J
中科院分区：
文献类型：
--
作者：
Lin Q;Wu F;Sheng P;Zhang Z;Zhang X;Guo X;Wang J;Cheng Z;Wang J;Wang H;Wan J
脱落酸（阿坝）和赤霉酸（GA）拮抗调节高等植物的许多发育过程和对生物或非生物胁迫的反应。然而，这种拮抗作用的分子机制仍然知之甚少。在这里，我们表明，水稻分蘖增强子（TE），APC/CTE复合物的激活剂，功能突变的损失，导致过敏和过敏症阿坝和GA，分别。我们发现TE与阿坝受体OsPYL/RCARs物理相互作用，并促进它们被蛋白酶体降解。遗传分析还表明OsPYL/RCAR在TE下游介导阿坝反应。相反，阿坝通过激活SNF 1相关蛋白激酶（SnRK 2）磷酸化TE来抑制APC/CTE活性，这可能会中断TE与OsPYL/RCAR之间的相互作用，从而稳定OsPYL/RCAR。相比之下，GA可以降低SnRK 2的水平，并且可以促进APC/CTE介导的OsPYL/RCAR的降解。因此，我们建议SnRK 2-APC/CTE调节模块代表了植物中GA和阿坝拮抗作用的调节中心。 激素脱落酸和赤霉素在植物发育和胁迫反应中起拮抗作用。在这里，Lin等人表明水稻分蘖增强蛋白是赤霉素诱导的脱落酸信号传导组分降解所必需的，揭示了对激素信号传导串扰的机理见解。
Abscisic acid (ABA) and gibberellic acid (GA) antagonistically regulate many developmental processes and responses to biotic or abiotic stresses in higher plants. However, the molecular mechanism underlying this antagonism is still poorly understood. Here, we show that loss-of-function mutation in rice Tiller Enhancer (TE), an activator of the APC/CTE complex, causes hypersensitivity and hyposensitivity to ABA and GA, respectively. We find that TE physically interacts with ABA receptor OsPYL/RCARs and promotes their degradation by the proteasome. Genetic analysis also shows OsPYL/RCARs act downstream of TE in mediating ABA responses. Conversely, ABA inhibits APC/CTE activity by phosphorylating TE through activating the SNF1-related protein kinases (SnRK2s), which may interrupt the interaction between TE and OsPYL/RCARs and subsequently stabilize OsPYL/RCARs. In contrast, GA can reduce the level of SnRK2s and may promote APC/CTE-mediated degradation of OsPYL/RCARs. Thus, we propose that the SnRK2-APC/CTE regulatory module represents a regulatory hub underlying the antagonistic action of GA and ABA in plants. The hormones abscisic acid and gibberellins act antagonistically in plant development and stress responses. Here Lin et al. show that the rice Tiller Enhancer protein is required for gibberellin-induced degradation of abscisic acid signalling components, uncovering mechanistic insights into hormone signalling crosstalk.