Epoxy-amino groups:: A new tool for improved immobilization of proteins by the epoxy method
Epoxy-amino groups:: A new tool for improved immobilization of proteins by the epoxy method
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DOI：
10.1021/bm0257661
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发表时间：
2003-05-01
影响因子：
6.2
通讯作者：
Fernández-Lafuente, R
中科院分区：
文献类型：
--
作者：
Mateo, C;Torres, R;Fernández-Lafuente, R
一种新的市售氨基环氧树脂载体（氨基环氧树脂-Sepabeads）的固定化酶的性能进行了比较，那些传统的环氧树脂支持。新载体在与载体共价结合的乙二胺层上具有环氧基团层。因此，该载体具有大的阴离子交换能力和高数量的环氧基。我们已经发现了这种新的异功能支持的许多优点。使用氨基环氧Sepabeads在低离子强度下进行固定，而使用常规单官能环氧载体需要高离子强度。使用氨基-环氧树脂载体的固定比使用常规环氧树脂载体快得多。获得固定化制剂的可能性，其中酶的取向可能不同于使用传统疏水载体获得的酶取向（可能影响活性或稳定性）。已经发现，在许多情况下，使用新载体的固定化酶的稳定性比使用常规载体的制备高得多。在这里，我们展示了使用不同酶（β-半乳糖苷酶，脂肪酶，戊二酰酰酰化酶，转化酶和葡糖淀粉酶）的这些优点的一些例子。
The properties of a new commercially available amino-epoxy support (amino-epoxy-Sepabeads) for immobilizing enzymes have been compared to those of conventional epoxy supports. The new support has a layer of epoxy groups over a layer of ethylenediamine that is covalently bound to the support. Thus, this support has a great anionic exchanger power and a high number of epoxy groups. We have found a number of advantages to this new heterofunctional support. Immobilization proceeds at low ionic strength using amino epoxy Sepabeads while requiring high ionic strength using conventional monofunctional epoxy supports. Immobilization is much more rapid using amino-epoxy supports than employing conventional epoxy supports. The possibility of achieving immobilized preparations in which the enzyme orientation may be different to that obtained using the traditional hydrophobic supports (with likely effects in terms of activity or stability). Stability of the immobilized enzyme has been found to be much higher using the new support than in preparations using the conventional ones in many cases. Here we show some examples of these advantages using different enzymes (beta-galactosidases, lipase, glutaryl acylase, invertase, and glucoamylase).