Environment and excavation: Pre-lab impacts on ancient DNA analyses
Environment and excavation: Pre-lab impacts on ancient DNA analyses
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DOI：
10.1016/j.crpv.2008.02.002
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发表时间：
2008-04-01
影响因子：
1.1
通讯作者：
Vigne, Jean-Denis
中科院分区：
文献类型：
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作者：
Bollongino, Ruth;Tresset, Anne;Vigne, Jean-Denis
古代DNA （aDNA）分析在古生物学、考古学和考古研究中发挥着越来越重要的作用。aDNA研究的限制因素是DNA保存的程度。对291具来自欧洲、近东和北非的史前牛遗骸的研究表明，DNA保存主要受地理和气候条件的影响。特别是在炎热的气候下，样品材料的保存率普遍较低。我们观察到这些标本在挖掘过程中和挖掘后容易进一步退化和污染。为了促进考古学家和遗传学家之间的合作，并改善未来研究的结果，我们给出了主要注意事项的描述和适当的样本处理的简短指南。
Ancient DNA (aDNA) analyses enjoy an increasing role in palaeontological, archaeological and archaeozoological research. The limiting factor for aDNA studies is the degree of DNA preservation. Our study on 291 prehistoric cattle remains from Europe, the Near East and North Africa revealed that DNA preservation is mainly influenced by geographic and climatic conditions. Especially in hot climates, the preservation of sample material is generally low. We observed that these specimens are prone to further degradation and contamination during and after excavation. We give a description of the main caveats and a short guideline for adequate sample handling in order to facilitate the cooperation between archaeologists and geneticists and to improve the outcome of future research.