Doxycycline induces dysbiosis in female C57BL/6NCrl mice.
Doxycycline induces dysbiosis in female C57BL/6NCrl mice.
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DOI：
10.1186/s13104-017-2960-7
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发表时间：
2017-11-29
影响因子：
1.8
通讯作者：
Wilkinson JE
中科院分区：
文献类型：
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作者：
Boynton FDD;Ericsson AC;Uchihashi M;Dunbar ML;Wilkinson JE
本研究旨在证明口服多西环素对小鼠粪便微生物群的影响。强力霉素是转基因小鼠中使用 tet 诱导系统控制基因表达的常见效应物。口服多西环素对小鼠肠道微生物群的影响尚未有报道。我们通过对 4 周治疗过程中收集的粪便样本中 16S rRNA 基因的 V4 高变区进行测序，评估了多西环素治疗的效果，剂量为 2 mg/ml 的饮用水。接受治疗的动物的粪便微生物群与对照动物不同；丰富度和多样性下降的主要特征是拟杆菌属。丰富。当暂时停止治疗 1 周后，这些影响仍然存在。这些数据表明，多西环素治疗可引起显着的生态失调，在对肠道微生物群扰动敏感或可能敏感的动物模型中使用时应考虑其影响。本文的在线版本 (10.1186/s13104-017-2960-7) 包含补充材料，可供授权用户使用。
This study aims to demonstrate the effect of oral doxycycline on fecal microbiota of mice. Doxycycline is a common effector for control of gene expression using the tet-inducible system in transgenic mice. The effect of oral doxycycline on murine gut microbiota has not been reported. We evaluated the effect of doxycycline treatment by sequencing the V4 hypervariable region of the 16S rRNA gene from fecal samples collected during a 4 week course of treatment at a dose of 2 mg/ml in the drinking water. The fecal microbiota of treated animals were distinct from control animals; the decreased richness and diversity were characterized primarily by Bacteroides sp. enrichment. These effects persisted when the treatment was temporarily discontinued for 1 week. These data suggest that doxycycline treatment can induce significant dysbiosis, and its effects should be considered when used in animal models that are or maybe sensitive to perturbation of the gut microbiota. The online version of this article (10.1186/s13104-017-2960-7) contains supplementary material, which is available to authorized users.