Study on Rapid Determination of Chlorophyll Content of Leaves
Study on Rapid Determination of Chlorophyll Content of Leaves
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发表时间：
2002
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通讯作者：
Yang Min
中科院分区：
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作者：
Yang Min
采用分光光度法研究了三种提取溶剂对测定叶片叶绿素含量的影响。结果表明，不同提取溶剂对叶绿素的吸收光谱相似。混合丙酮-乙醇作为叶绿素的提取溶剂，具有提取速度快、提取效率高、稳定性好、测定精度高等特点。并提出了一种快速测定叶绿素含量的方法。
Effect of three extracting solvents on the determination of chlorophyll contents of leaves was studied by spectrophotometric method. The results show that chlorophyll absorption spectrums are similar in different extracting solvents. Mixed acetone and ethanol as extracting solvent of chlorophyll has the peculiarity of a quick extract speed, high extract efficiency, good stability and determination accuracy. And a method of rapid determination of chlorophyll content is proposed.