THE ROLE OF BRADYKININ IN MEDIATING ISCHEMIC BRAIN EDEMA IN RATS
THE ROLE OF BRADYKININ IN MEDIATING ISCHEMIC BRAIN EDEMA IN RATS
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DOI：
10.1161/01.str.24.4.571
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发表时间：
1993-04-01
期刊：
影响因子：
8.3
通讯作者：
TERASHI, A
中科院分区：
文献类型：
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作者：
KAMIYA, T;KATAYAMA, Y;TERASHI, A
背景和目的：我们探讨了缓激肽的产生可能导致自发性高血压大鼠缺血性脑水肿的假说。方法：67只大鼠通过双侧颈总动脉夹闭造成脑缺血3h。在缺血期后，对大鼠进行再灌注。测量脑含水量和能量代谢产物（三磷酸腺苷、乳酸和丙酮酸）以及血浆和组织缓激肽。此外，使用相同的实验范式，在缺血诱导后立即给予缓激肽合成抑制剂（抑肽酶[n=7]和大豆胰蛋白酶抑制剂[n=7]），以确定缓激肽生成与缺血性脑水肿进展的关系。脑含水量在缺血3小时内增加，在再灌注30分钟时达到峰值，此后下降。血浆和组织中的缓激肽水平在再灌注后30分钟显著升高，此后下降。三磷酸腺苷的进行性损失反映在乳酸的上升。在治疗组中，给予抑肽酶和大豆胰蛋白酶抑制剂显著减轻脑水肿（p
Background and Purpose: We investigated the hypothesis that bradykinin generation may induce ischemic brain edema in spontaneously hypertensive rats.Methods: Cerebral ischemia lasting 3 hours was produced by bilateral common carotid artery occlusion in 67 rats. After the ischemic period, the rats were reperfused. Cerebral water content and energy metabolites (adenosine triphosphate, lactate, and pyruvate), as well as plasma and tissue bradykinin, were measured. Additionally, using the same experimental paradigm, bradykinin synthesis inhibitors (aprotinin [n=7] and soybean trypsin inhibitor [n=7]) were administered immediately after ischemia induction to determine the relation of bradykinin generation to the progression of ischemic brain edema.Results. Cerebral water content increased during the 3-hour ischemic period, peaked at 30 minutes of reperfusion, and declined thereafter. Bradykinin levels in plasma and tissue rose markedly 30 minutes after reperfusion and fell thereafter. The progressive loss of adenosine triphosphate was mirrored by the rise in lactate. In the treated groups, aprotinin and soybean trypsin inhibitor administration significantly attenuated cerebral edema (p