Müller cell phenotype exhibited by senescent RSV-transformed chicken neuroretinal cells.
Müller cell phenotype exhibited by senescent RSV-transformed chicken neuroretinal cells.
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衰老 RSV 转化鸡神经视网膜细胞表现出的 Müller 细胞表型。
DOI：
10.1007/bf02634542
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Notter,MF
中科院分区：
文献类型：
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作者：
Seigel,GM;Hansen,JT;Imperato,EL;Notter,MF
编辑先生：成功获得永久建立的Rous肉瘤病毒(RSV)感染的鸡细胞株用于长期研究，经常受到长期培养后自发退化和死亡的现象的阻碍(10)。来自7日龄胚胎鸡的神经视网膜细胞培养含有视网膜神经元细胞和神经胶质(Miiller)细胞的混合物，这些细胞在体外具有分化能力，但在有限数量的细胞分裂后停止增殖[2]。虽然感染RSV(8)可以刺激体外的进一步增殖，但这种影响通常是暂时的。在我们试图建立RSV感染的鸡神经视网膜细胞的连续细胞系的过程中(13)，我们的团队记录了在四个月的时间里在鸡神经视网膜细胞培养LA29NR中失去有丝分裂活性和转化的Pb型，其中包括在第15代细胞死亡之前出现异常的形态。在第15代(p15+)保持两周的LA29NR细胞表现为大的扁平细胞，其中一些(约30%)发出突起(图1B)。这种程度的突起生长和细胞大小不是早期传代LA29NR的典型(图1A)。此外，尽管p15+LA29NR具有衰老的性质，但仍然存在逆转录病毒颗粒(图2B)。这与我们之前的结果(13)一致，表明后期传代的LA29NR表达逆转录病毒蛋白pp60 TM，并继续显示pp60 TM的几个潜在底物的酪氨酸磷酸化。超微结构上，p15+LA29NR细胞表现出与Miiller细胞表型一致的特征(2，19)。直径约5到10纳米的纵向排列的中间丝在细胞的大部分都可见，在突起中尤其明显(图2B)。鸡Miiller细胞的一个显著特征是存在myehn图形，我们在LA29NR(图2 63)中观察到了这一点，并与体内正常的鸡Miiller细胞相关联进行了描述(11)。在这方面，LA29NR填充物细胞似乎与体内的鸡Miiller相似
Dear Editor: Success in obtaining permanently established Rous sarcoma virus (RSV)-infected chicken cell lines for long-term studies has often been hindered by a phenomenon of spontaneous degeneration and death after extended periods in culture (10). Neuroretinal cell cultures derived from Day 7 embryonic chicken contain a mixture of both neuronal cells and glial (Miiller) cells of the retina which have the capacity to differentiate in vitro, but cease to proliferate after a limited number of cell divisions (2). Although further proliferation in vitro can be stimulated by infection with RSV (8), this effect is usually temporary (10). During our attempts to establish a continuous cell line of RSV-infected chick neuroretinal ceils (13), our group documented the loss of mitotic activity and transformed pbenotype in the chick neuroretinal cell culture LA29NR over a period of four months, which included the appearance of unusual morphologies immediately prior to cell death at Passage 15. We now report on the characterization of these unusual senescent cells. LA29NR cells maintained at Passage 15 for two weeks (p15+) appeared as large flat cells, some of which (approximately 30%) sent out processes (Fig. 1 B). This extent of process outgrowth and cell size was not typical of early passage LA29NR (Fig. 1 A). Also, despite the senescent nature of p15+ LA29NR, there was a continued presence of retroviral particles (Fig. 2 A). This was consistent with our previous results (13) which demonstrated that late passage LA29NR expressed the retroviral protein pp60 TM, and continued to exhibit tyrosine phosphorylation of several potential substrates of pp60 TM.Ultrastrueturally, p15+ LA29NR cells displayed features consistent with a Miiller cell phenotype (2, 19). Longitudinally-packed intermediate filaments of approximately 5 to 10 nm in diameter were visible throughout much of the cell, and were especially prominent within processes (Fig. 2 B). One distinguishing feature of chicken Miiller cells is the presence of myehn figures, which we observed in LA29NR (Fig. 2 63, and are described in association with normal chicken Miiller cells in vivo (11). In this respect, LA29NR Mfiller cells appeared to resemble in vivo chicken Miiller