2D and 3D liver models.
2D and 3D liver models.
复制标题
2D 和 3D 肝脏模型。
DOI：
10.1016/j.jhep.2022.06.022
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发表时间：
2023
影响因子：
25.7
通讯作者：
Stevens,KellyR
中科院分区：
文献类型：
--
作者：
Saxton,SarahH;Stevens,KellyR
肝脏为人体执行数百种基本功能，包括蛋白质合成，氮代谢，解毒和药物代谢。然而，肝细胞，肝脏的主要功能细胞，历来难以离体培养。为了克服这一障碍，已经引入了各种互补的体外肝脏模型。这些模型可以大致分为2D和3D模型，现在提供了一个相当大的工具包，用于研究人类肝脏生物学和疾病。培养形式限制和肝功能（例如，白蛋白分泌、细胞色素诱导和尿素分泌）对每个模型进行分层，尽管它们都不简单易用，也不能有效预测所有肝功能。未来的工作必须集中在模型的可负担性/可访问性，吞吐量和广泛的肝功能的长期维持。
The liver performs hundreds of essential functions for the human body, including protein synthesis, nitrogen metabolism, detoxification, and drug metabolism. Yet hepatocytes, the primary functional cell of the liver, have been historically difficult to culture ex vivo. To overcome this hurdle, a variety of complementary in vitro liver models have been introduced. These models, which can be broadly categorized into 2D vs. 3D models, now offer a sizable toolkit with which to study human hepatic biology and disease. Culture format restrictions and hepatic functionality (eg, albumin secretion, cytochrome induction, and urea secretion) stratify each model, though none of them are both simple to use and effective for predicting all hepatic functions. Future work must focus on model affordability/accessibility, throughput, and long-term maintenance of a broad range of hepatic functions.