Use of dyes to investigate migration of the chiral selector in CFFE and the impact on the chiral separations.
Use of dyes to investigate migration of the chiral selector in CFFE and the impact on the chiral separations.
复制标题
使用染料研究 CFFE 中手性选择剂的迁移以及对手性分离的影响。
DOI：
10.1021/ac010220a
复制
发表时间：
2001
影响因子：
7.4
通讯作者：
Stalcup,AM
中科院分区：
文献类型：
--
作者：
Gratz,SR;Schneiderman,E;Mertens,TR;Stalcup,AM
研究了连续自由流动电泳作为一种使用硫酸化 β-环糊精 (sβ-CD) 作为手性添加剂来制备哌罗生对映体手性分离的工具。通过对收集的各个组分进行 LC-MS 分析，并通过向分离缓冲液中添加结晶紫进行可视化，证实了 sβ-CD 的大量迁移。在不存在 sβ-CD 的情况下，含有结晶紫的缓冲液呈红色/紫色，并且结晶紫在腔室中发生阴极偏转。在 sβ-CD 存在下，含有结晶紫的缓冲液呈蓝色并发生阳极偏转。然而，在这两种情况下，积累区和消耗区的形成都很明显。在阴极清洗溶液中添加 sβ-CD 可以几乎完全解析哌罗生对映体，处理速率为 0.45 mg/h。
Continuous free flow electrophoresis was investigated as a tool for the preparative chiral separation of piperoxan enantiomers using sulfated β-cyclodextrin (sβ-CD) as the chiral additive. Bulk migration of sβ-CD was confirmed using LC−MS analysis of the individual fractions collected and visualized with the addition of crystal violet to the separation buffer. In the absence of sβ-CD, the crystal violet-containing buffer was reddish/purple and the crystal violet was deflected cathodically in the chamber. In the presence of sβ-CD, the crystal violet-containing buffer was blue and was deflected anodically. However, formation of accumulation and depletion zones was apparent in both cases. The addition of sβ-CD to the cathodic wash solution allowed for almost complete resolution of the piperoxan enantiomers with a processing rate of 0.45 mg/h.