THE P150 AND P60 SUBUNITS OF CHROMATIN ASSEMBLY FACTOR-I - A MOLECULAR LINK BETWEEN NEWLY SYNTHESIZED HISTONES AND DNA-REPLICATION
THE P150 AND P60 SUBUNITS OF CHROMATIN ASSEMBLY FACTOR-I - A MOLECULAR LINK BETWEEN NEWLY SYNTHESIZED HISTONES AND DNA-REPLICATION
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DOI：
10.1016/s0092-8674(05)80015-7
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发表时间：
1995-06-30
期刊：
影响因子：
64.5
通讯作者：
STILLMAN, B
中科院分区：
文献类型：
--
作者：
KAUFMAN, PD;KOBAYASHI, R;STILLMAN, B
染色质组装因子I（Chromatin assembly factor I，CAF-I）是一种由三个亚基组成的蛋白质复合物，在无细胞体系中将组蛋白八聚体组装到复制DNA上。编码两个最大的CAF-I亚基的cDNA序列显示，p150蛋白含有大簇的带电残基，而p60含有WD重复。p150和p60直接相互作用，并且都是核小体的DNA复制依赖性组装所必需的。从p150蛋白中删除p60结合结构域可阻止染色质组装。p150和p60在人类细胞提取物中与新合成的组蛋白H3和乙酰化的H4形成复合物，表明这种复合物是组蛋白合成和组装到复制DNA上之间的中间体。
Chromatin assembly factor I (CAF-I) from human cell nuclei is a three-subunit protein complex that assembles histone octamers onto replicating DNA in a cell-free system. Sequences of cDNAs encoding the two largest CAF-I subunits reveal that the p150 protein contains large clusters of charged residues, whereas p60 contains WD repeats. p150 and p60 directly interact and are both required for DNA replication-dependent assembly of nucleosomes. Deletion of the p60-binding domain from the p150 protein prevents chromatin assembly. p150 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in human cell extracts, suggesting that such complexes are intermediates between histone synthesis and assembly onto replicating DNA.