Evidence for stacking interactions between 5-mercurated polyuridylic acid and HIV-1 p7 nucleocapsid protein obtained by phosphorescence and optically detected magnetic resonance (ODMR).
Evidence for stacking interactions between 5-mercurated polyuridylic acid and HIV-1 p7 nucleocapsid protein obtained by phosphorescence and optically detected magnetic resonance (ODMR).
复制标题
通过磷光和光学检测磁共振 (ODMR) 获得 5-汞多尿苷酸和 HIV-1 p7 核衣壳蛋白之间堆积相互作用的证据。
DOI：
10.1016/0014-5793(93)80962-t
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发表时间：
1993
期刊：
影响因子：
3.5
通讯作者：
Henderson,LE
中科院分区：
文献类型：
--
作者：
Lam,WC;Maki,AH;Casas-Finet,JR;Erickson,JW;Sowder2nd,RC;Henderson,LE
The photoexcited triplet state of Trp-37 in the C-terminal zinc finger of the HIV-1 p7 nucleocapsid protein was used as a probe of p7 interactions with the heavy atom-derivatized RNA homopolymer, poly-5-mercuriuridylic acid (5-HgU). Binding of p7 to 5-HgU (Hg blocked with 2-mercaptoethanol) produces an external heavy atom effect (HAE) on Trp-37 characterized by fluorescence quenching, reduction of the phosphorescence lifetime by three orders of magnitude, and the appearance of the D + E phosphorescence-detected ODMR signal, absent in unperturbed Trp, but induced by a HAE. The details of the HAE are consistent with out-of-plane van der Waals contact of Hg with the indole chromophore of Trp-37. Steric requirements suggest further that the Trp-RNA contact occurs via an aromatic stacking interaction.
