Human herpesvirus 8 infection in patients with POEMS syndrome-associated multicentric Castleman's disease
Human herpesvirus 8 infection in patients with POEMS syndrome-associated multicentric Castleman's disease
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DOI：
10.1182/blood.v93.11.3643.411k38_3643_3653
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发表时间：
1999-06-01
期刊：
影响因子：
20.3
通讯作者：
Gherardi, RK
中科院分区：
文献类型：
--
作者：
Bélec, L;Mohamed, AS;Gherardi, RK
多发性神经病、器官肿大、内分泌病、M 蛋白、皮肤变化 (POEMS) 综合征是一种罕见的多系统性疾病，与骨硬化性骨髓瘤和多中心卡斯尔曼病 (MCD) 相关。约 40% 人类免疫缺陷病毒 (HIV) 阴性 MCD 患者的淋巴结以及多发性骨髓瘤患者的骨髓基质细胞中检测到了人类疱疹病毒 8 型 (HHV-8) DNA 序列。考虑到这些数据，我们调查了 18 名 POEMS 综合征患者（9 名患有 MCD）中是否存在 HHV-8，通过巢式聚合酶链反应 (N-PCR) 检测活检或尸检时获得的各种细胞和组织中的 DNA 序列（13 名患者，其中 7 名患有 MCD），并通过免疫荧光测定法检测血液中的抗 HHV-8 IgG 抗体（18 名患者，其中 9 名患有 MCD）。使用三种不同的 N-PCR 检测 HHV-8 DNA，针对开放阅读框 (ORF) 25 和 ORF26 中的非重叠区域。通过所有三种 N-PCR 评估，13 名 POEMS 患者中的 7 名 (54%) 其组织中含有 HHV-8 DNA 序列，18 名 POEMS 患者中的 9 名 (50%) 具有循环抗 HHV-8 抗体。 HHV-8 主要在患有 MCD 的 POEMS 患者亚组中检测到（7 例中的 6 例 [85%] 检测到 DNA 序列；9 例中的 7 例检测到抗体 [78%]）。淋巴结中 N-PCR 阳性的百分比高于骨髓样本中的阳性率 (P
The polyneuropathy, organomegaly, endocrinopathy, M protein, skin changes (POEMS) syndrome is a rare multisystemic disorder associated with osteosclerotic myeloma and multicentric Castleman's disease (MCD). Human herpesvirus type 8 (HHV-8) DNA sequences have been detected in lymph nodes of about 40% of human immunodeficiency virus (HIV)-negative patients with MCD, and in bone marrow stromal cells of patients with multiple myeloma. Considering these data, we investigated the presence of HHV-8 in 18 patients with POEMS syndrome (9 with MCD), by nested polymerase chain reaction (N-PCR) to detect DNA sequenses in various cells and tissues obtained by biopsy or at autopsy (13 patients, of whom 7 had MCD), and by an immunofluorescence assay to detect anti-HHV-8 IgG antibodies in blood (18 patients, of whom 9 had MCD). Detection of HHV-8 DNA was performed using three different N-PCR, targeting nonoverlapping regions in open reading frame (ORF) 25 and ORF26. Seven of 13 (54%) POEMS patients had HHV-8 DNA sequences in their tissues, as assessed by all three N-PCR, and 9 of 18 (50%) had circulating anti-HHV-8 antibodies. HHV-8 was mainly detected in the subset of POEMS patients with MCD (6 of 7 [85%] for DNA sequences; 7 of 9 [78%] for antibodies). The percentage of positive N-PCR was higher in lymph nodes than in bone marrow samples (P