Increased expression of integrin alpha3beta1 in highly brain metastatic subclone of a human non-small cell lung cancer cell line.
Increased expression of integrin alpha3beta1 in highly brain metastatic subclone of a human non-small cell lung cancer cell line.
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DOI：
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发表时间：
2004
期刊：
影响因子：
5.7
通讯作者：
T. Yoshimasu;T. Sakurai;S. Oura;I. Hirai;H. Tanino;Y. Kokawa;Y. Naito;Y. Okamura;I. Ota;
中科院分区：
文献类型：
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作者：
T. Yoshimasu;T. Sakurai;S. Oura;I. Hirai;H. Tanino;Y. Kokawa;Y. Naito;Y. Okamura;I. Ota;
为阐明整合素和细胞外基质（ECM）在非小细胞肺癌（NSCLC）脑转移过程中的作用，建立了人NSCLC细胞系EBC-1/original裸鼠体内脑转移模型，并建立了高脑转移亚克隆EBC-1/brain和高骨转移亚克隆EBC-1/bone。通过流式细胞术评估这些亚克隆的整合素表达。使用这些亚克隆进行了ECM的体外细胞附着、迁移和增殖试验。整合素α 3亚基在EBC-1/脑中的表达高于EBC-1/原始和EBC-1/骨。体外细胞粘附、迁移和增殖试验表明，EBC-1/脑比EBC-1/原始和EBC-1/骨对层粘连蛋白具有更高的亲和力和更高的反应性。阻断整合素α 3 β 1可显著降低EBC-1/brain的脑转移（P < 0.05）。整合素α 3 β 1与层粘连蛋白的相互作用在非小细胞肺癌脑转移过程中起重要作用。
To clarify the roles of integrin and extracellular matrix (ECM) in the process of non-small cell lung cancer (NSCLC) brain metastasis, we established an in vivo model of brain metastasis of human NSCLC cell line EBC-1/original in athymic mice, and established highly brain metastatic subclone EBC-1/brain and highly bone metastatic subclone EBC-1/bone. Integrin expression of these subclones was evaluated by flow cytometry. In vitro cell attachment, migration and proliferation assays with ECMs were performed using these subclones. Expression of integrin alpha3 subunit was higher in EBC-1/brain than in both EBC-1/original and EBC-1/bone. In vitro cell attachment, migration, and proliferation assays revealed that EBC-1/brain had higher affinity and higher reactivity to laminin than EBC-1/original and EBC-1/bone. Blocking of integrin alpha3beta1 significantly (P < 0.05) decreased brain metastasis by EBC-1/brain. Interaction of integrin alpha3beta1 and laminin plays important roles in the process of brain metastasis of non-small cell lung cancer.