Transgene as vaccine for chlamydia
Transgene as vaccine for chlamydia
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DOI：
10.1016/s0002-8703(99)70291-7
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发表时间：
1999-11-01
影响因子：
4.8
通讯作者：
Zhang, DJ
中科院分区：
文献类型：
--
作者：
Brunham, RC;Zhang, DJ
背景我们评估了用沙眼衣原体小鼠肺炎（MoPn）株的主要外膜蛋白（MOMP）基因DNA免疫诱导对衣原体感染的保护性免疫的潜在效用。方法和结果Balb/c小鼠组用裸DNA肌肉内或鼻内或用MOMP DNA转染的鼠伤寒沙门氏菌口服免疫。通过肺途径用MoPn攻击小鼠以测定保护性免疫。3种途径的DNA免疫均能引起保护性免疫。结论衣原体MOMP基因DNA免疫可用于疫苗的研制。
Background We evaluated the potential utility of DNA immunization with the major outer membrane protein (MOMP) gene of Chlamydia trachomatis mouse pneumonitis (MoPn) strain for induction of protective immunity to chlamydial infection in mice.Methods and Results Groups of Balb/c mice were immunized with naked DNA intramuscularly or intranasally or with MOMP DNA-transfected Salmonella typhimurium delivery orally. Mice were challenged with MoPn through the pulmonary route to assay for protective immunity. All 3 routes of DNA immunization elicited protective immunity. Mucosal delivery appeared more efficacious than intramuscular delivery.Conclusions DNA immunization with the chlamydia MOMP gene may be suitable for vaccine development.