The detection of differentially expressed microRNAs from the serum of ovarian cancer patients using a novel real-time PCR platform
The detection of differentially expressed microRNAs from the serum of ovarian cancer patients using a novel real-time PCR platform
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DOI：
10.1016/j.ygyno.2008.08.036
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发表时间：
2009-01-01
影响因子：
4.7
通讯作者：
Cohn, David E.
中科院分区：
文献类型：
--
作者：
Resnick, Kimberly E.;Alder, Hansjuerg;Cohn, David E.
Objective.确定血清miRNAs作为上皮性卵巢癌生物标志物的效用。从组织和血清库中确定了28例经组织学证实的上皮性卵巢癌患者。在确定性治疗前收集血清。使用15个不匹配的健康对照进行比较。从所有患者中获得血清。使用单步Trizol方法的衍生物提取RNA。使用TaqMan Array Human MicroRNA panel，通过实时PCR将9份癌症标本的RNA与4份正常标本进行比较。21个miRNAs在正常和患者血清中差异表达。对19例癌组织和11例正常组织进行实时荧光定量PCR检测。使用比较C-t方法，在最初的21种mRNA中鉴定出8种在癌症和正常标本之间显著差异表达。与对照组相比，癌症患者血清中的miRNAs-21、92、93、126和29 a显著过表达（p
Objective. To determine the utility of serum miRNAs as biomarkers for epithelial ovarian cancer.Methods. Twenty-eight patients with histologically confirmed epithelial ovarian cancer were identified from a tissue and serum bank. Serum was collected prior to definitive therapy. Fifteen unmatched, healthy controls were used for comparison. Serum was obtained from all patients. RNA was extracted using a derivation of the single step Trizol method. The RNA from 9 cancer specimens was compared to 4 normal specimens with real-time PCR using the TaqMan Array Human MicroRNA panel. Twenty-one miRNAs were differentially expressed between normal and patient serum. Real-time PCR for the 21 individual miRNAs was performed on the remaining 19 cancer specimens and 11 normal specimens.Results. Eight mRNAs of the original twenty-one were identified that were significantly differentially expressed between cancer and normal specimens using the comparative C-t method. MiRNAs-21, 92, 93, 126 and 29a were significantly over-expressed in the serum from cancer patients compared to controls (p