A 13 kb resolution cosmid map of the 14 Mb fission yeast genome by nonrandom sequence-tagged site mapping.
A 13 kb resolution cosmid map of the 14 Mb fission yeast genome by nonrandom sequence-tagged site mapping.
复制标题
通过非随机序列标记位点作图绘制 14 Mb 裂殖酵母基因组的 13 kb 分辨率粘粒图谱。
DOI：
10.1016/0092-8674(93)90165-m
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发表时间：
1993
期刊：
影响因子：
64.5
通讯作者：
Marr,TG
中科院分区：
文献类型：
--
作者：
Mizukami,T;Chang,WI;Garkavtsev,I;Kaplan,N;Lombardi,D;Matsumoto,T;Niwa,O;Kounosu,A;Yanagida,M;Marr,TG
我们提出了一种非随机序列标记位点（STS）含量检测方法在绘制裂变酵母全基因组图谱中的应用。我们策略的新颖之处在于使用从世界克隆的两端制成的STS探针，根据“不替换样本”选择（只有没有显示先前阳性杂交的文库克隆被选择并制成探针）。我们开发了基于一致性分析的强大技术，用于错误检测和配置组装。此外，我们用遗传标记和Notl或sll连接克隆探测了我们的文库，从而将contigs锚定在染色体上。我们的图谱包含超过1000个位点，包括基因（大多数以前未绘制）、已知重复元件的发生和Notl-Sfil限制性位点。
We present the application of a nonrandom sequencetagged site (STS) content detection method in mapping an entire genome, that of fission yeast. The novelty of our strategy is in the use of STS probes made from both endsof cosmld clones, selected on the basis of “sample without replacement”(only library clones that show no previous positive hybridization are selected and made into probes). We developed powerful techniques, based on consistency analysis, for error detection and contig assembly. In addition, we probed our library with genetically mapped markers and Notl or Sfll linking clones, thereby anchoring contigs onto chromosomes. Our map contains more than 1000 sites, including genes (most were previously unmapped), occurrences of known repetitive elements, and Notl-Sfil restriction sites.