Bacterial aerosols in dental practice - a potential hospital infection problem?
Bacterial aerosols in dental practice - a potential hospital infection problem?
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DOI：
10.1016/j.jhin.2006.04.011
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发表时间：
2006-09
期刊：
影响因子：
--
通讯作者：
Meurman JH
中科院分区：
文献类型：
--
作者：
Rautemaa R;Nordberg A;Wuolijoki-Saaristo K;Meurman JH
在恢复性牙科中使用现代高速旋转仪器时，从患者口腔中产生含有微生物的气溶胶。随着口腔-鼻腔耐甲氧西林金黄色葡萄球菌定植患者数量的增加，这些气溶胶在牙科手术中传播的范围和污染程度日益受到关注。目前的研究旨在确定在牙科治疗过程中空气传播细菌的程度以及污染程度。在离患者0.5-2 m处放置6个不同部位的血琼脂板上收集落屑样本。从使用高速旋转器械的房间（N = 6）收集牙科修复放射性尘埃样本（N = 72），从不使用旋转和超声器械的牙周和正畸治疗房间（N = 4）收集对照样本（N = 24）。采集时间分别为1.5 h和3 h，并分别从消毒前和消毒后的人员口罩和房间表面采集样本。37℃孵育48 h后，用革兰氏染色法对菌落进行计数和分类。结果显示，当使用高速仪器时，在所有距离取样的房间都有明显的污染（平均970菌落形成单位/m2/h）。越远的采样点细菌密度越高。革兰氏阳性球菌，即翠绿链球菌和葡萄球菌是最常见的发现。在牙科手术过程中被污染的区域比以前想象的要大得多，实际上包括了整个房间。这些结果强调需要开发新的手段来防止微生物气溶胶在牙科和保护所有物品暂时存放在工作面上。在医院环境中治疗一般病人或免疫功能低下的病人时，这一点尤为重要。
Aerosols containing microbes from the oral cavity of the patient are created when using modern high-speed rotating instruments in restorative dentistry. How far these aerosols spread and what level of contamination they cause in the dental surgery has become a growing concern as the number of patients with oro-nasal meticillin-resistant Staphylococcus aureus colonization has increased. The present study aimed to determine how far airborne bacteria spread during dental treatment, and the level of contamination. Fall out samples were collected on blood agar plates placed in six different sectors, 0.5–2 m from the patient. Restorative dentistry fallout samples (N = 72) were collected from rooms (N = 6) where high-speed rotating instruments were used, and control samples (N = 24) were collected from rooms (N = 4) used for periodontal and orthodontic treatment where rotating and ultrasonic instruments were not used. The collection times were 1.5 and 3 h. In addition, samples were taken from facial masks of personnel and from surfaces in the rooms before and after disinfection. After 48 h of incubation at 37 °C, colonies were counted and classified by Gram stain. The results showed significant contamination of the room at all distances sampled when high-speed instruments were used (mean 970 colony-forming units/m2/h). The bacterial density was found to be higher in the more remote sampling points. Gram-positive cocci, namely viridans streptococci and staphylococci, were the most common findings. The area that becomes contaminated during dental procedures is far larger than previously thought and practically encompasses the whole room. These results emphasize the need for developing new means for preventing microbial aerosols in dentistry and protection of all items stored temporarily on work surfaces. This is especially important when treating generally ill or immunocompromised patients at dental surgeries in hospital environments.
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影响因子：
6.9
作者：
Smith, A;Letters, S;Bagg, J
通讯作者：
Bagg, J
DOI：
10.14219/jada.archive.1995.0111
发表时间：
1995-12-01
影响因子：
3.9
作者：
LOGOTHETIS, DD;MARTINEZWELLES, JM
通讯作者：
MARTINEZWELLES, JM
DOI：
10.14219/jada.archive.1994.0093
发表时间：
1994-05-01
影响因子：
3.9
作者：
BENTLEY, CD;BURKHART, NW;CRAWFORD, JJ
通讯作者：
CRAWFORD, JJ
影响因子：
3.3
作者：
Kedjarune, U;Kukiattrakoon, B;Leggat, P
通讯作者：
Leggat, P
影响因子：
7.6
作者：
MICIK, RE;MILLER, RL;RYGE, G
通讯作者：
RYGE, G