Genetic and molecular characterization of P element-induced mutations reveals that the Drosophila ovarian tumor gene has maternal activity and a variable null phenotype.
Genetic and molecular characterization of P element-induced mutations reveals that the Drosophila ovarian tumor gene has maternal activity and a variable null phenotype.
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P 元件诱导突变的遗传和分子特征表明，果蝇卵巢肿瘤基因具有母体活性和可变的无效表型。
DOI：
10.1093/genetics/133.2.265
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发表时间：
1993
期刊：
影响因子：
3.3
通讯作者：
Nagoshi,RN
中科院分区：
文献类型：
--
作者：
Geyer,PK;Patton,JS;Rodesch,C;Nagoshi,RN
卵巢肿瘤（otu）基因的突变使卵子发生在发育的几个阶段停止。对 otu 区域的一系列缺失突变进行了表征，每种突变都会导致 otu 转录物的缺失或减少。这些等位基因的范围从最严重的突变（导致卵巢缺乏卵囊）到相对轻微的突变（允许晚期卵母细胞发育）。这些突变的异等位基因组合表明，otu 突变体卵巢的表型复杂性是由于 otu 活性的剂量依赖性要求造成的。互交和发育性 Northern 印迹研究表明，雌性种系发育过程中母体对 otu 的需求。此外，我们证明otu合子无效表型是可变的，从最极端情况下不存在包囊到存在肿瘤卵室。
The mutations in the ovarian tumor (otu) gene arrest oogenesis at several stages in development. A series of deletion mutations in the otu region were characterized, each of which causes the absence or reduction of the otu transcript. These alleles range from the most severe class, which results in ovaries lacking egg cysts, to relatively mild mutations that allow the development of late stage oocytes. Heteroallelic combinations of these mutations demonstrate that the phenotypic complexity of otu mutant ovaries is due to a dosage dependent requirement for otu activity. Reciprocal cross and developmental Northern blot studies suggest a maternal requirement for otu in the development of the female germline. In addition we demonstrate that the otu zygotic null phenotype is variable, ranging from the absence of cysts in the most extreme cases, to the presence of tumorous egg chambers.