Rapid diagnostics for melioidosis: a comparative study of a novel lateral flow antigen detection assay
Rapid diagnostics for melioidosis: a comparative study of a novel lateral flow antigen detection assay
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DOI：
10.1099/jmm.0.000098
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发表时间：
2015-08-01
影响因子：
3
通讯作者：
Norton, Robert
中科院分区：
文献类型：
--
作者：
Robertson, Gemma;Sorenson, Alanna;Norton, Robert
败血症类鼻疽的快速诊断将对降低死亡率产生影响。目前，这几乎完全依赖于从临床样品中培养病原体类鼻疽伯克霍尔德氏菌。在急性脓毒症中，血液是用于培养的优选样本，因此应该是快速诊断工具的目标。用于检测B的侧流免疫测定（LFI）。已经开发出了类鼻疽抗原。这与使用靶T3SS1和IpxO的分子检测进行了比较。45个EDTA血液，这是培养阳性的临床样本，使用这两种方式进行了测试。LFI的灵敏度为40%，而分子检测的灵敏度为20%。先前已经描述了分子检测的不良性能，并且在很大程度上与使用收集到含有EDTA的血液管中的全血样本有关。虽然不理想，但LFI将是类鼻疽快速诊断的辅助手段。
The rapid diagnosis of septicaemic melioidosis will have an impact on reduction of mortality. Currently, this relies almost exclusively upon culture of the causative agent Burkholderia pseudomallei from clinical samples. In acute sepsis, blood is the preferred specimen for culture and therefore should be the target for a rapid diagnostic tool. A lateral flow immunoassay (LFI) for the detection of B. pseudomallei antigen has been developed. This was compared with molecular detection using the targets T3SS1 and IpxO. Forty-five clinical samples of EDTA blood, which were culture-positive, were tested using both modalities. The LFI had a sensitivity of 40 %, whilst molecular detection had a sensitivity of 20 %. The poor performance of molecular detection has been described previously and is largely related to the use of whole-blood specimens collected into blood tubes containing EDTA. Whilst suboptimal, the LFI would be an adjunct in the rapid diagnosis of melioidosis.