Functional Characterization of the Apple RING E3 Ligase MdMIEL1 in Transgenic Arabidopsis
Functional Characterization of the Apple RING E3 Ligase MdMIEL1 in Transgenic Arabidopsis
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DOI：
10.1016/j.hpj.2017.01.001
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发表时间：
2017-03-01
影响因子：
5.7
通讯作者：
Hao Yujin
中科院分区：
文献类型：
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作者：
An Jianping;Liu Xin;Hao Yujin
E3泛素连接酶参与多种生理过程，在生长发育中发挥着关键作用。在这项研究中，我们在苹果果实 (Malus xdomestica) 中发现了一个以前未知的基因，并将其命名为 MdMIEL1。 MdMIEL1 基因编码的蛋白质在其 N 末端含有锌指结构域，在 C 末端含有环指基序。为了研究 MdMIEL1 的功能，我们生成了在花椰菜花叶病毒 35S 启动子控制下表达 MdMIEL1 基因的转基因拟南芥系。有趣的是，MdMIEL1在拟南芥中的异位表达产生了多种表型，包括相对于野生型植物的早发芽、早开花和侧根数量增加。进一步分析表明，MdMIEL1 通过增加根中生长素的积累来调节侧根的形成。总之，这些结果表明，MdMIEL1作为一种新型环指泛素连接酶影响植物的生长发育，并强调MdMIEL1调节侧根生长。
E3 ubiquitin ligases are involved in various physiological processes, and they play pivotal roles in growth and development. In this study, we identified a previously unknown gene in the apple fruit (Malus xdomestica) and named it MdMIEL1. The MdMIEL1 gene encoded a protein that contained a zinc-finger domain at its N-terminus and a RING-finger motif at its C-terminus. To investigate MdMIEL1 functions, we generated transgenic Arabidopsis lines expressing the MdMIEL1 gene under the control of the Cauliflower mosaic virus 35S promoter. Interestingly, ectopic expression of MdMIEL1 in Arabidopsis produced multiple phenotypes, including early germination, early flowering and a lateral root number increase relative to wild-type plants. Further analysis indicated that MdMIEL1 regulated lateral root initiation by increasing auxin accumulation in the roots. In a word, these results suggest that, MdMIEL1 as a novel RING-finger ubiquitin ligase influences plant growth and development, and highlight that MdMIEL1 regulates lateral root growth.