Inhibitory effects of the anesthetics propofol and sevoflurane on spontaneous lymphatic vessel activity in rats
Inhibitory effects of the anesthetics propofol and sevoflurane on spontaneous lymphatic vessel activity in rats
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DOI：
10.1097/00000542-200409000-00017
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发表时间：
2004-09-01
期刊：
影响因子：
8.8
通讯作者：
Namiki, A
中科院分区：
文献类型：
--
作者：
Hattori, J;Yamakage, M;Namiki, A
背景：异丙酚和七氟醚对淋巴管活性的影响尚不清楚。本研究旨在阐明这些麻醉药对大鼠淋巴管活性的影响，通过使用机械去除内皮的技术。方法：首先用染色剂注射大鼠股骨区，观察异丙酚（8mg /kg）和七氟醚（2.0%）对体内淋巴流量的影响。在体外研究中，大鼠胸导管血管段的两端分别连接注射器和旋塞。监测每个节段直径的自发变化，并将每个节段的腔外侧暴露于异丙酚（1 × 10（-6）类似于3 × 10(-5) M）或七氟醚（0.5类似于2.0%）。通过向管腔灌注空气消除了内皮功能。结果：在染料摄取研究中，对照组80%的髂淋巴结呈阳性染色，而异丙酚组和七氟醚组分别只有10%和20%呈阳性染色。在体外研究中，两种麻醉剂都显著降低了具有或不具有内源性功能的淋巴管的自发活动幅度。七氟醚抑制淋巴管活动频率，异丙酚对其无影响。当内源性功能消除时，两种麻醉剂都降低了淋巴管自发活动的频率。结论：异丙酚和七氟醚对内皮功能的影响可能有所不同，其调节淋巴管自发收缩的起搏。
Background: The effects of propofol and sevoflurane on lymphatic vessel activity are unknown. This study aimed to clarify the effects of these anesthetics on lymphatic vessel activity in rats by the use of a technique for mechanical removal of the endothelium.Methods: The authors first examined the effects of propofol (8 mg/kg) and sevoflurane (2.0%) on in vivo lymphatic flow by injection of dye into the femoral regions of rats. In the in vitro study, the ends of the vessel segments of rat thoracic duct were connected to a syringe and stopcock, respectively. Spontaneous changes in diameter of each segment were monitored, and the extraluminal side of each segment was exposed to propofol (1 x 10(-6) similar to 3 x 10(-5) M) or sevoflurane (0.5similar to2.0%). Endothelial function was eliminated by perfusion of air into the lumen.Results: In the dye uptake study, 80% of iliac lymphatic nodes were positively stained in a control group, whereas only 10% and 20% were positively stained in propofol and sevoflurane groups, respectively. in the in vitro study, both of the anesthetics significantly decreased the amplitude of spontaneous activity of lymphatic vessels with or without endothetial function. Sevolflurane inhibited the frequency of lymphatic vessel activity but propofol had no effect on it. When the endothetial function was eliminated, both anesthetics decreased the frequency of spontaneous activity of lymphatic vessels.Conclusions: Propofol and sevoflurane seem to have some different effects on endothelial function, which regulates the pacemaking of spontaneous contraction of lymphatic vessels.