Evolution of microRNA genes by inverted duplication of target gene sequences in Arabidopsis thaliana
Evolution of microRNA genes by inverted duplication of target gene sequences in Arabidopsis thaliana
复制标题
DOI：
10.1038/ng1478
复制
发表时间：
2004-12-01
期刊：
影响因子：
30.8
通讯作者：
Carrington, JC
中科院分区：
文献类型：
--
作者：
Allen, E;Xie, ZX;Carrington, JC
植物和动物中的MicroRNAs （miRNAs）是靶基因的转录后调控因子，其中许多参与多细胞发育。mirna通过碱基对互补引导效应复合物靶向mrna，促进位点特异性切割或翻译抑制。mirna的生物发生涉及具有广泛折叠结构的前体转录物的核裂变加工。在这里，我们提供的证据表明，在植物中，编码miRNAs的基因起源于靶基因序列的反向复制。在拟南芥和其他小rna产生位点中，一些最近进化的编码mirna的基因具有倒置复制事件的特征，这些事件形成了各自折叠前体两侧的臂。我们提出了一个miRNA进化模型，该模型提出了具有独特靶标特异性的新miRNA基因从头生成的机制。
MicroRNAs (miRNAs) in plants and animals function as post-transcriptional regulators of target genes, many of which are involved in multicellular development. miRNAs guide effector complexes to target mRNAs through base-pair complementarity, facilitating site-specific cleavage or translational repression. Biogenesis of miRNAs involves nucleolytic processing of a precursor transcript with extensive foldback structure. Here, we provide evidence that genes encoding miRNAs in plants originated by inverted duplication of target gene sequences. Several recently evolved genes encoding miRNAs in Arabidopsis thaliana and other small RNA-generating loci possess the hallmarks of inverted duplication events that formed the arms on each side of their respective foldback precursors. We propose a model for miRNA evolution that suggests a mechanism for de novo generation of new miRNA genes with unique target specificities.