Simulating Illumina metagenomic data with InSilicoSeq.
Simulating Illumina metagenomic data with InSilicoSeq.
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DOI:
10.1093/bioinformatics/bty630
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发表时间:
2019-02-01
期刊:
影响因子:
--
通讯作者:
Bongcam-Rudloff E
中科院分区:
文献类型:
--
作者:
Gourlé H;Karlsson-Lindsjö O;Hayer J;Bongcam-Rudloff E
The accurate in silico simulation of metagenomic datasets is of great importance for benchmarking bioinformatics tools as well as for experimental design. Users are dependant on large-scale simulation to not only design experiments and new projects but also for accurate estimation of computational needs within a project. Unfortunately, most current read simulators are either not suited for metagenomics, out of date or relatively poorly documented. In this article, we describe InSilicoSeq, a software package to simulate metagenomic Illumina sequencing data. InsilicoSeq has a simple command-line interface and extensive documentation. InSilicoSeq is implemented in Python and capable of simulating realistic Illumina (meta) genomic data in a parallel fashion with sensible default parameters. Source code and documentation are available under the MIT license at https://github.com/HadrienG/InSilicoSeq and https://insilicoseq.readthedocs.io/. Supplementary data are available at Bioinformatics online.
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