Experimental self-etching HEMA-free adhesive systems: cytotoxicity and degree of conversion
Experimental self-etching HEMA-free adhesive systems: cytotoxicity and degree of conversion
复制标题
DOI：
10.1007/s10856-014-5370-6
复制
发表时间：
2015-01-01
影响因子：
3.7
通讯作者：
da Silva, Adriana Fernandes
中科院分区：
文献类型：
--
作者：
Barbosa, Marilia Oliveira;de Carvalho, Rodrigo Varella;da Silva, Adriana Fernandes
本研究的目的是评估甲基丙烯酸酯表面活性剂单体取代甲基丙烯酸2-羟乙酯（HEMA）对两步自酸蚀牙本质粘合剂系统的细胞毒性和转化程度的影响。测试了五种不含HEMA的粘合剂系统：Bis-EMA 10、Bis-EMA 30、PEG 400、PEG 400 UDMA、PEG 1000，并且使用HEMA组作为阳性对照。使用鼠成纤维细胞系3 T3和四唑测定（3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四唑（MTT））评估具有不同单体浓度（2或20重量%）的实验引物和含有2,5重量%表面活性剂的粘合树脂的细胞毒性。使用傅里叶变换红外光谱分析粘结树脂的转化度。使用设定为P < 0.05的显著性水平对数据进行统计分析。与2%引物中的HEMA相比，PEG 1000组获得更高的细胞活力。使用20%引物的细胞存活率表明PEG 1000和BIS-EMA 10的细胞毒性低于HEMA。关于粘合树脂的降解，数据显示BIS-EMA 10、BIS-EMA 30和PEG 400 UDMA组的细胞毒性低于HEMA。实验组和HEMA组的转化程度之间无统计学显著差异。PEG 1000、BIS-EMA 10和30单体显示出用于新型粘合剂系统制剂的生物学潜力，因为与含HEMA组相比，它们显示出较低的细胞毒性和相似的转化程度。
The aim of this study was to evaluate the effect of replacing 2-hydroxyethyl methacrylate (HEMA) by methacrylate surfactant monomers on the cytotoxicity and degree of conversion of two-step self-etching dentin adhesive systems. Five HEMA-free adhesive systems were tested: Bis-EMA10, Bis-EMA 30, PEG400, PEG400UDMA, PEG1000, and a HEMA group was used as positive control. The cytotoxicity of the experimental primers, with different monomer concentrations (2 or 20 wt%), and bond resins, containing 25 wt% surfactant, was assessed using murine fibroblast cell line 3T3 and the tetrazolium assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)). The degree of conversion of the bond resins was analyzed using Fourier transform infrared spectroscopy. The data were submitted to statistical analysis using level of significance set at P < 0.05. The PEG 1000 group obtained higher cell viability in comparison with HEMA in the 2 % primer. The cell survival rate using 20 % primer showed that PEG1000 and BIS-EMA 10 were less cytotoxic than HEMA. With regard to the eluate from bond resin, the data showed that the groups BIS-EMA 10, BIS-EMA 30 and PEG400UDMA were less cytotoxic than HEMA. No statistically significant difference was found among degrees of conversion of the experimental groups and HEMA. PEG 1000, BIS-EMA 10 and 30 monomers showed the biological potential for use in new adhesive system formulations since they showed lower cytotoxicity and similar degree of conversion when compared with the HEMA-containing group.