Microfabricated monolithic multinozzle emitters for nanoelectrospray mass spectrometry
Microfabricated monolithic multinozzle emitters for nanoelectrospray mass spectrometry
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DOI：
10.1021/ac070010j
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发表时间：
2007-05-15
影响因子：
7.4
通讯作者：
Wang, Daojing
中科院分区：
文献类型：
--
作者：
Kim, Woong;Guo, Mingquan;Wang, Daojing
质谱分析是蛋白质组学的关键技术。为了充分实现蛋白质组学芯片实验室的巨大潜力，需要在微流体与质谱的接口方面取得重大进展。在这里，我们报告的第一个演示的单片集成的多喷嘴电喷雾发射器与微流体通道通过一种新的硅微加工工艺。这些微加工的单片多喷嘴发射器（M-3发射器）可以很容易地从硅晶片大规模生产。每个发射器由一个平行的二氧化硅喷嘴阵列组成，喷嘴阵列从一个中空的硅条中伸出，导管尺寸为100 × 10 μ m。独立式喷嘴的尺寸和数量可以在制造过程中系统地和精确地控制。一旦与质谱仪集成，M-3发射器在肽和蛋白质检测中实现了与商业二氧化硅基毛细管纳米电喷雾尖端相当的灵敏度和稳定性。这些M-3发射器可以发挥作用，作为一个完全集成的硅/硅基微全分析系统的蛋白质组学的关键组成部分。
Mass spectrometry is the enabling technology for proteomics. To fully realize the enormous potential of lab-on-a-chip in proteomics, a major advance in interfacing microfluidics with mass spectrometry is needed. Here, we report the first demonstration of monolithic integration of multinozzle electrospray emitters with a microfluidic channel via a novel silicon microfabrication process. These microfabricated monolithic multinozzle emitters (M-3 emitters) can be readily mass-produced from silicon wafers. Each emitter consists of a parallel silica nozzle array protruding out from a hollow silicon sliver with a conduit size of 100 x 10 mu m. The dimension and number of freestanding nozzles can be systematically and precisely controlled during the fabrication process. Once integrated with a mass spectrometer, M-3 emitters achieved sensitivity and stability in peptide and protein detection comparable to those of commercial silica-based capillary nanoelectrospray tips. These M-3 emitters may play a role as a critical component in a fully integrated silicon/silica-based micro total analysis system for proteomics.