Proportions of Mycobacterium massiliense and Mycobacterium bolletii strains among Korean Mycobacterium chelonae-Mycobacterium abscessus group isolates
Proportions of Mycobacterium massiliense and Mycobacterium bolletii strains among Korean Mycobacterium chelonae-Mycobacterium abscessus group isolates
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DOI：
10.1128/jcm.00319-08
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发表时间：
2008-10-01
影响因子：
9.4
通讯作者：
Kook, Yoon-Hoh
中科院分区：
文献类型：
--
作者：
Kim, Hee-Youn;Kook, Yoonwon;Kook, Yoon-Hoh
通过PCR限制性分析（PRA）和16 S rRNA基因、rpoB和hsp 65的比较序列分析，对从韩国两家不同医院分离的龟分枝杆菌-结核分枝杆菌组的韩国分离株进行鉴定，以评估4种密切相关物种（M.龟类、龟类M.脓肿M. Escherichiense和M. bolletii）。144株快速生长分枝杆菌中，127株（88.2%）属于M。龟类M. - 是的在这一组中，M。龟鳖M. M.P. Escherichiense和M.博莱氏菌分别占0.8%（n = 1）、51.2%（n = 65）、46.5%（n = 59）和1.6%（n = 2）。结果不一致的两株分离株为M.通过rpoB序列分析和M.经hsp 65序列分析，最终鉴定为M.基于对sodA和16 S-23 S内转录间隔区的额外分析，M.以前用hsp 65 PRA鉴定的马立克次氏菌群I分离物均为M。而组II分离物被进一步鉴定为M.或M.通过对rpoB和hsp 65进行测序，光滑、粗糙或混合菌落。P.观察到了伊氏菌分离物。M.对克拉霉素高度耐药的利什曼原虫菌株在23 S rRNA基因的肽基转移酶区域的第2058位（A（2058））或第2059位（A（2059））的腺嘌呤处存在点突变。
Korean isolates of the Mycobacterium chelonae-Mycobacterium abscessus group, which had been isolated from two different hospitals in South Korea, were identified by PCR restriction analysis (PRA) and comparative sequence analysis of 16S rRNA genes, rpoB, and hsp65 to evaluate the proportion of four closely related species (M. chelonae, M. abscessus, M. massiliense, and M. bolletii). Of the 144 rapidly growing mycobacterial strains tested, 127 strains (88.2%) belonged to the M. chelonae-M. abscessus group. In this group, M. chelonae, M. abscessus, M. massiliense, and M. bolletii accounted for 0.8% (n = 1), 51.2% (n = 65), 46.5% (n = 59), and 1.6% (n = 2), respectively. Two isolates which showed discordant results, M. massiliense by rpoB sequence analysis and M. abscessus by hsp65 sequence analysis, were finally identified as M. massiliense based on the additional analysis of sodA and the 16S-23S internal transcribed spacer. M. abscessus group I isolates previously identified by hsp65 PRA were all found to be M. abscessus, whereas group II isolates were further identified as M. massiliense or M. bolletii by sequencing of rpoB and hsp65. Smooth, rough, or mixed colonies of both M. abscessus and M. massiliense isolates were observed. M. massiliense strains that were highly resistant to clarithromycin had a point mutation at the adenine at position 2058 (A(2058)) or 2059 (A(2059)) in the peptidyltransferase region of the 23S rRNA gene.