UV radiation elevates arylalkylamine N-acetyltransferase activity and melatonin content in the two-spotted spider mite, Tetranychus urticae.
UV radiation elevates arylalkylamine N-acetyltransferase activity and melatonin content in the two-spotted spider mite, Tetranychus urticae.
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DOI：
10.1016/j.jinsphys.2008.06.005
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发表时间：
2008-07
影响因子：
2.2
通讯作者：
Takeshi Suzuki;Takuya Takashima;Norimitsu Izawa;Masakatsu Watanabe;M. Takeda
中科院分区：
文献类型：
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作者：
Takeshi Suzuki;Takuya Takashima;Norimitsu Izawa;Masakatsu Watanabe;M. Takeda
紫外线 (UV) 辐射会在哺乳动物体内产生活性氧 (ROS)，其中褪黑激素起着 ROS 清除剂的作用。褪黑激素合成酶芳基烷基胺 N-乙酰转移酶 (NAT) 是可能的 ROS 去除系统中的重要元素。使用日本冈崎国家基础生物学研究所的冈崎大型光谱仪，通过单色光照射二斑叶螨，测定 NAT 活性和褪黑激素含量的变化。蓝光（450nm）抑制 NAT 活性和褪黑激素含量。没有观察到红光（650nm）对 NAT 活性和褪黑激素含量的影响。紫外线辐射对 NAT 活性和褪黑激素含量具有强度依赖性的双重影响。在UV-B（300nm）处理中，NAT活性和褪黑激素含量在低于1μmolm−2s−1的强度下受到抑制，但在强度高达10μmolm−2s−1时升高。在UV-A（350nm）处理中，当强度高达10μmolm−2s−1时，褪黑激素含量升高，而在强度低于10和1μmolm−2s−1时，NAT活性和褪黑激素含量分别受到抑制。高强度紫外线照射导致 NAT 活性和褪黑激素含量升高，可能表明紫外线信号启动了褪黑激素合成，以去除螨虫中的 ROS。
Ultraviolet (UV) radiation produces reactive oxygen species (ROS) in mammals, where melatonin plays the role of a ROS scavenger. The melatonin synthetic enzyme arylalkylamine N-acetyltransferase (NAT) is a significant element in a possible ROS removal system. Changes in NAT activity and melatonin content were determined in the two-spotted spider mite Tetranychus urticae by irradiating it with monochromatic light using the Okazaki Large Spectrograph at the National Institute for Basic Biology, Okazaki, Japan. The NAT activity and melatonin content were suppressed by blue light (450nm). No effects of red light (650nm) on the NAT activity and melatonin content were observed. UV radiation had intensity-dependent dual effects on the NAT activity and melatonin content. In the UV-B (300nm) treatment, the NAT activity and melatonin content were suppressed at the intensity below 1μmolm−2s−1but elevated when the intensity was as high as 10μmolm−2s−1. In the UV-A (350nm) treatment, the melatonin content was elevated when the intensity was as high as 10μmolm−2s−1, though the NAT activity and melatonin content were suppressed at the intensity below 10 and 1μmolm−2s−1, respectively. Elevation of the NAT activity and melatonin content by high intensity UV irradiation may indicate that the UV signals initiate melatonin synthesis for ROS removal in mites.