p16INK4A is a surrogate biomarker for a subset of human papilloma virus-associated dysplasias of the uterine cervix as determined on the Pap smear
p16INK4A is a surrogate biomarker for a subset of human papilloma virus-associated dysplasias of the uterine cervix as determined on the Pap smear
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DOI：
10.1002/dc.20175
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发表时间：
2005-01-01
影响因子：
1.3
通讯作者：
Youssef, E
中科院分区：
文献类型：
--
作者：
Bose, S;Evans, H;Youssef, E
最近，p16（INK 4A）已被确定为人类乳头状瘤病毒（HPV）诱导的子宫颈发育异常病变的生物标志物，并已提出，它可能是一个有用的辅助诊断这些病变。因此，进行本研究以确定p16表达在液体培养基中收集的一系列巴氏（Pap）涂片中的效用，并确定其相对于HPV检测的益处。对107例宫颈癌组织进行p16蛋白表达和HPV DNA检测，其中23例为阴性，34例为低度鳞状上皮内病变（LSIL），16例为高度鳞状上皮内病变（HSIL），29例为意义不明的非典型鳞状细胞（ASC-US），5例为ASC-H。我们观察到p16蛋白表达在所有细胞学异常的病例中仅占36%（30/84），在所有与高危型HPV相关的病例中占40%。在HSIL中观察到最高的阳性率（80%）和最高的表达水平（超过3 - 5个阳性细胞/10 X视野）。在ASC-H病例中观察到类似结果。这表明，在可疑的情况下，p16可以用于确诊。另一方面，p16阳性仅见于21%的LSIL和ASC-US病例。这提出了一个有趣的可能性，鉴于只有少数LSIL病例进展到更高级别的病变，p16可能有助于对这些患者进行更密切的随访和/或进一步评估。需要进一步的研究进行确认。(C)2005 Wiley-Liss，Inc.
Recently, p16(INK4A) has been identified as a biomarker for human papilloma virus (HPV)-induced dysplastic lesions of the cervix and it has been suggested that it may be a useful diagnostic aid for these lesions. This study therefore was performed to determine the utility of p16 expression in a series of Papanicolaou (Pap) smears collected in liquid medium and to determine its benefit, if an),, over HPV testing. One hundred seven cases, including 23 negative cases, 34 with low-grade squamous intraepithelial lesion (LSIL), 16 with high-grade squamous intraepithelial lesion (HSIL), 29 with atypical squamous cells of uncertain significance (ASC-US), and 5 cases with ASC suspicious for HSIL (ASC-H), were evaluated for both p16 expression and HPV DNA. We observed p16 expression ire only 36% of all cases with abnormal cytology (30/84) and in 40% of all cases associated with high-risk HPV. The highest rate of positivity (80%) and the highest levels of expression (more than three to five positive cells/10X field) were seen in HSIL. Similar results were observed with ASC-H cases. This suggests that in equivocal cases, p16 may be used,for confirmation of the diagnosis. On the other hand, p16 positivity was noted in only 21% of LSIL and ASC-US cases. This raises the interesting possibility, given that only a minority of LSIL cases progress on to higher-grade lesions, that p16 might be useful for triaging these patients for closer follow-up and/or,further evaluation. Additional studies are required for confirmation. (C) 2005 Wiley-Liss, Inc.